| RP-HPLC-PAD法测定薄荷中乌索酸和齐墩果酸的含量 |
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| 引用本文： | 邹盛勤,陈武.RP-HPLC-PAD法测定薄荷中乌索酸和齐墩果酸的含量[J].福建林学院学报,2008,28(1):73-76. |
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| 作者姓名： | 邹盛勤 陈武 |
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| 作者单位： | 宜春学院生物工程研究所,江西省天然药物活性成分研究重点实验室,江西,宜春,336000 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 建立反相高效液相色谱法测定薄荷中乌索酸和齐墩果酸的含量。采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm)和二极管阵列检测器,流动相为甲醇∶水∶磷酸(90∶10∶0.2),流速0.8 mL.min-1,柱温28℃,检测波长210 nm。乌索酸进样量0.67-6.03μg(r=0.999 62),齐墩果酸进样量0.66-5.94μg(r=0.999 65),具有良好的线性关系。乌索酸和齐墩果酸平均回收率分别为97.6%、99.8%,RSD分别为1.6%、2.1%。方法简便、准确,重现性好,线性范围宽,可用于同时测定薄荷中乌索酸和齐墩果酸的含量。
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| 关 键 词： | 薄荷 反相高效液相色谱法 乌索酸 齐墩果酸 二极管阵列检测器 |
| 文章编号： | 1001-389X(2008)01-0073-04 |
| 修稿时间： | 2007年5月31日 |
Determination of ursolic acid and oleanolic acid in Mentha Haplocalyxby RP-HPLC with photodiode array detector |
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| ZOU Sheng-qin,CHEN Wu.Determination of ursolic acid and oleanolic acid in Mentha Haplocalyxby RP-HPLC with photodiode array detector[J].Journal of Fujian College of Forestry,2008,28(1):73-76. |
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| Authors: | ZOU Sheng-qin CHEN Wu |
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| Abstract: | A new HPLC method for determination of ursolic acid and oleanolic acid in Mentha haplocalyx was developed.The Kromasil C18(250 mm×4.6 mm,5 μm) was used as analytical column with methanol-water-phosphate(90∶10∶0.2) as mobile phase.The detection wavelength was set at 210 nm with photodiode array detector.The flow rate was 0.8 mL·min-1 and the column temperature was 28 ℃.The calibration curve of ursolic acid was linear over the range of 0.67-6.03 μg,and the correlation coefficient was 0.999 62.The calibration curve of oleanolic acid was linear over the range of 0.66-5.94 μg,and the correlation coefficient was 0.999 65.The average recoveries of ursolic acid and oleanolic acid were 97.6%(RSD=1.6%) and 99.8%(RSD=2.1%) respectively.The method is simple,accurate and reproducible.It has wide linear range and can be used for the simultaneous determination of ursolic acid and oleanolic acid in M.haplocalyx. |
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| Keywords: | Mentha haplocalyx RP-HPLC ursolic acid oleanolic acid photodiode array detector |
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