| 四唑染色法测定厚朴种子生活力的条件研究 |
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| 引用本文： | 石磊,徐广灿,杨红兵.四唑染色法测定厚朴种子生活力的条件研究[J].安徽农业科学,2013,41(13):5706-5706,5730. |
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| 作者姓名： | 石磊 徐广灿 杨红兵 |
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| 作者单位： | 湖北中医药大学药学院,湖北武汉,430061;湖北中医药大学药学院,湖北武汉,430061;湖北中医药大学药学院,湖北武汉,430061 |
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| 基金项目： | 湖北省教育厅科研重点项目 |
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| 摘 要： | 目的]探讨厚朴种子生活力的快速测定方法。方法]采用四唑染色法(TTC法),设置不同的TTC浓度、染色温度和时间,研究厚朴种子生活力测定的适宜条件。结果]TTC法测定厚朴种子生活力的适宜条件为:采用浓度0.2%的TTC溶液,在55℃的水浴中染色50 min。结论]该法可作为厚朴种子生活力测定的有效方法。
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| 关 键 词： | 厚朴种子 TTC法 种子生活力 |
Study on the Determination Conditions of Seed Viability of Magnolia officinalis Rehd.et Wils.by TTC Method |
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| Institution: | SHI Lei et al ( School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065 ) |
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| Abstract: | Objective] To study on rapid determination method of seed viability of Magnolia officinalis Rehd. et Wils. Method] Using TTC method and setting different TTC concentration, dyeing temperature and dyeing time, the suitable conditions of Magnolia officinalis seed viabil- ity determination were studied. Result] The optimal determination conditions for the viability of Magnolia officinalis seed is that staining for 50min in 55 ℃ water bath with 0.5% Trc solution. Conclusion] This is an effective method to be used to determine Magnolia officinalis seed viability. |
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| Keywords: | Magnolia officinalis Rehd et Wils TTC method Seed viability |
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