| RP-HPLC法测定陆英叶中绿原酸含量的研究 |
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| 引用本文： | 刘传安,邹盛勤.RP-HPLC法测定陆英叶中绿原酸含量的研究[J].安徽农业科学,2005,33(12):2334-2335. |
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| 作者姓名： | 刘传安 邹盛勤 |
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| 作者单位： | 宜春学院理学院,江西,宜春,336000;宜春学院生物工程研究所,江西省天然药物活性成分研究重点实验室,江西,宜春,336000 |
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| 摘 要： | 建立反相高效液相色谱法测定了陆英叶中绿原酸的含量,同时采用光电二极管阵列检测器检测绿原酸色谱峰的纯度。色谱柱为Nova-PakC18(3.9 mm×150mm,4μm),流动相为水-甲醇-乙酸(881∶21∶,V/V),流速1.0ml/min,检测波长326nm,柱温25℃。绿原酸在0.832～4.16μg范围内线性关系良好(r=0.99992),检出限为0.4mg/L(3σ),平均回收率为97.1%。该法简便、准确、重现性好、线性范围宽,适于对含有绿原酸的药材进行质量评价。
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| 关 键 词： | 陆英 绿原酸 光电二极管阵列检测器 反相高效液相色谱 含量测定 |
| 文章编号： | 0517-6611（2005）12-2334-02 |
| 收稿时间： | 10 18 2005 12:00AM |
| 修稿时间： | 2005-10-18 |
Determination of Chlorogenic Acid in Sambucus chinensis Lindl. Leaf by RP- HPLC |
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| LIU Chuan-an et al.Determination of Chlorogenic Acid in Sambucus chinensis Lindl. Leaf by RP- HPLC[J].Journal of Anhui Agricultural Sciences,2005,33(12):2334-2335. |
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| Authors: | LIU Chuan-an |
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| Abstract: | A determination method of chlorogenic acid in Sambucus chinensis Lindl.leaf by reversed-phase high performance liquid chromtograph was established and at the time the purity of chlorogenic acid peak was detected by means of Photodiode Array Detector(PAD).The chromatographic column,Nova-Pak C18(3.9 mm×150 mm,4 μm),water-methanol-acetic acid(88∶12∶1,V/V)mobile phase with 1.0 ml/min flow rate,the detected wavelength(326 nm) and the column temperature(25 ℃) were adopted.The calibration curve of chlorogenic acid was linear under the content of 0.832 ～4.16 μg,the correlation coefficient was over 0.999 92 and the detected limit was 0.4 mg/L(3σ).The average recovery of chlorogenic acid was 97.1 %.The method can be adapted to evaluate the quality of medicine that contains chlorogenic acid. |
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| Keywords: | Sambucus chinensis Lindl Chlorogenic acid Photodiode array detector RP- HPLC Content determination |
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