| 长白山区珍稀濒危植物野生玫瑰植株再生体系的建立 |
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| 引用本文： | 杨丽娟,顾地周,秦莉,何雪梅.长白山区珍稀濒危植物野生玫瑰植株再生体系的建立[J].林业科学研究,2010,23(4):626-628. |
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| 作者姓名： | 杨丽娟 顾地周 秦莉 何雪梅 |
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| 作者单位： | 1. 通化师范学院生物系,吉林,通化,134002
2. 吉林省通化市林场,吉林,通化,134001 |
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| 基金项目： | 吉林省科技厅资助项目(200705C05) |
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| 摘 要： | 玫瑰(Rosa rugosa Thunb.)又称玫瑰花,是蔷薇科 (Rosaceae) 蔷薇属(Rosa L.)落叶灌木.该种为国家三级重点保护植物.<中国珍稀濒危保护植物名录(第一册)>中定为濒危种,<中国物种红色名录>定为易危种1],<吉林省野生动植物保护管理暂行条例>中定其为省级一类重点保护植物.是待开发的野生珍稀濒危药用植物,花、果、根入药,主治吐血、痛经、咯血、乳痈、月经不调等症.该种玫瑰具有喜光,耐寒,耐贫瘠,抗风等特性,可防止高山苔原带的水土流失,对维护生态平衡,美化天池环境以及引种驯化、开发利用于作护坡保土、园林绿化和培育玫瑰新品种;对研究该属植物的系统发育与地理分布也有重要科学价值.玫瑰在长白山区数量非常稀少,仅在珲春市敬信乡有零星分布.近年来,由于旅游设施及道路的修建等人为因素的干扰,其生境遭到严重破坏.又因玫瑰的天然更新能力非常弱,其种子成熟度小,种子繁殖可操作性极差,插条繁殖生根率和移栽成活率也极低,使其开发及利用受到极大限制.因此,在保护好现有野生资源的同时,本研究以玫瑰新生嫩茎为外植体,利用植物组织培养方法,采用均匀设计法,以期筛选出玫瑰嫩茎离体培养各阶段的培养基.目前,与其同属的其他种植物的离体培养已有报道,但关于该种玫瑰植株再生体系的研究迄今未见.
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| 关 键 词： | 玫瑰 离体培养 均匀设计 |
Plant Regeneration System of Rare and Endangered Wild Plant Rosa rugosa in Mountain Changbai |
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| YANG Li-juan,GU Di-zhou,QIN Li and HE Xue-mei.Plant Regeneration System of Rare and Endangered Wild Plant Rosa rugosa in Mountain Changbai[J].Forest Research,2010,23(4):626-628. |
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| Authors: | YANG Li-juan GU Di-zhou QIN Li and HE Xue-mei |
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| Institution: | Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin, China;;Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin, China;;Forest Farm of Tonghua City, Jilin Province,Tonghua 134001, Jilin, China);Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin, China; |
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| Abstract: | The tender stem of Rosa rugosa was used as explant and it suitable medium compositions were screened through uniform design experiments. The results showed that the tissue culture of Rosa rugosa required different kinds of culture medium in different phases. The most suitable culture media were as follows: B5+6-BA 2.90 mg·L-1 for shoots regeneration immediately at base of tender stem, the rate of regeneration was 96%; 1/2 MS(macro-elemenyts)+IBA 0.07 mg·L-1 for rooting, the rooting rate was more than 98.5%. Plant regeneration system of Rosa rugosa had been successfully established. |
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| Keywords: | Rosa rugosa in vitro culture uniform design |
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