| 几种果实总RNA提取方法的评价 |
| |
| 引用本文： | 陈弟,符贤英,殷晓敏,张荣意.几种果实总RNA提取方法的评价[J].广东农业科学,2007(11):30-33. |
| |
| 作者姓名： | 陈弟 符贤英 殷晓敏 张荣意 |
| |
| 作者单位： | 海南大学环境与植物保护学院,海南,儋州,571737 |
| |
| 基金项目： | 华南热带农业大学博士启动基金 |
| |
| 摘 要： | RNA的提取是分子生物学的基本内容之一,高质量的RNA是进行基因克隆、基因表达等研究的必要前提.针对果实中多酚、多糖和次生代谢产物等含量较高的特点,综述了试剂盒法、热硼酸法、异硫氰酸胍法、十二烷基硫磺酸钠法、十六烷基三甲基溴化铵法及其改进方法的原理、特点和在果实RNA提取上的应用,并对不同的提取方法进行了比较.
|
| 关 键 词： | 果实 RNA 提取方法 |
| 文章编号： | 1004-574X（2007）11-0030-04 |
| 修稿时间： | 2007-09-29 |
Assessment of several extraction methods of total RNA from fruits |
| |
| CHEN Di,FU Xian-ying,YIN Xiao-min,ZHENG Rong-yi.Assessment of several extraction methods of total RNA from fruits[J].Guangdong Agricultural Sciences,2007(11):30-33. |
| |
| Authors: | CHEN Di FU Xian-ying YIN Xiao-min ZHENG Rong-yi |
| |
| Institution: | College of Environment and Plant Protection, SCUTA, Danzhou 571737, China |
| |
| Abstract: | The extraction of total RNA is one of the elementary contents for molecular biology.Isolation of high-quality RNA is the necessary premise of gene cloning and gene expression.Because of high content of polyphenols,polysaccharide and secondary metabolites in fruits,this paper summarize the principle and characteristics of commercial Kit method,GT method,SDS method,CTAB method and their modified methods.Furthermore,it also introduce and compare these methods's application on the extraction of fruit RNA. |
| |
| Keywords: | fruit RNA extraction method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
