| Dc-Chol/DOPE介导siRNA对HeLa细胞的高效转染 |
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| 引用本文： | 党颖,王应雄,朱旭东,郭威威,王宇新,黄培堂.Dc-Chol/DOPE介导siRNA对HeLa细胞的高效转染[J].西南农业大学学报,2005,27(5):569-572. |
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| 作者姓名： | 党颖 王应雄 朱旭东 郭威威 王宇新 黄培堂 |
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| 作者单位： | [1]军事医学科学院生物工程研究所,北京100071 [2]重庆医科大学公共卫生学院,重庆400016 |
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| 基金项目： | 国家973计划（2002 CB 513204）和科技部（2004 AA 002100）资助课题 |
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| 摘 要： | 如何把小干涉RNA有效地递送到体内特异的靶细胞是RNA干涉技术发展中的主要障碍。阳离子脂质体DC-Chol／DOPE已经成功地用于DNA药物的体内递送。研究表明DC-Chol／DOPE介导siRNA对HeLa细胞的转染与商品化的转染试剂Lipofectamine 2000一样高效，而对细胞的毒性则要小得多。
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| 关 键 词： | 脂质体 转染 小干涉RNA |
| 文章编号： | 1000-2642（2005）05-0569-04 |
| 收稿时间： | 2005-01-27 |
| 修稿时间： | 2005-01-27 |
LIPOSOME DC - Chol/DOPE - MEDIATED SMALL INTERFERING RNA TRANSFECTION OF HeLa CELLS |
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| DANG Ying ,WANG Ying- xiong, ZHU Xu -dong , GUO Wei -wei , WANG Yu -xin , HUANG Pei -tang.LIPOSOME DC - Chol/DOPE - MEDIATED SMALL INTERFERING RNA TRANSFECTION OF HeLa CELLS[J].Journal of Southwest Agricultural University,2005,27(5):569-572. |
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| Authors: | DANG Ying WANG Ying- xiong ZHU Xu -dong GUO Wei -wei WANG Yu -xin HUANG Pei -tang |
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| Institution: | 1. Beijing Insatute of Biotechnology, Beijing 100071 ;2. Chongqing University of Medical Science, Chongqing 400016 |
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| Abstract: | The major obstacle to the use, of RNA interference is the need for efficient delivery of small interfering RNA into specific target ceils in vivo. Cationic liposome DC - Chol/DOPE has been successfully used in DNA medicine delivery. This research showed that the DC - Chol/DOPE transfected siRNA into HeLa ceils as effectively as commercial transfection reagent Lipofectamine 2000 and was much less toxic to cells. |
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| Keywords: | liposome transfection small interfering RNA |
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