| 杨树种质SSR指纹数据库构建 |
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| 引用本文： | 刘超凡,张国君,徐刚标.杨树种质SSR指纹数据库构建[J].中南林业科技大学学报,2021(2). |
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| 作者姓名： | 刘超凡 张国君 徐刚标 |
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| 作者单位： | 中南林业科技大学林学院;湖南省泰格林纸集团有限公司 |
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| 基金项目： | 国家“十三五”重点研发计划课题(2017YFD0601203)。 |
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| 摘 要： | 【目的】构建林木种质资源指纹数据库是林木种质材料遗传鉴定的前提,也可为林木杂交育种提供清晰的遗传学背景。【方法】选取亲缘关系较远的8份杨树无性系材料筛选SSR引物,基于TP-M13-SSR技术进行SSR-PCR扩增,采用ABI3730XL毛细管电泳系统检测PCR扩增产物,利用GeneMarkerV1.91进行基因分型,Cervus3.0.7估算多态信息含量(PIC)和各位SSR位点无效等位基因频率(pN),POPGENE3.2估算各SSR位点上的等位基因数目(Na)、Shannon信息指数(I)和观测杂合度(HO),NTSYS-pc2.1进行遗传聚类分析。【结果】共筛选出13对条带清晰、重复性好的SSR引物,共扩增出89条多态性条带,单个位点上的等位基因数目为2~12个,平均值为6.5个;Shannon’s信息指数(I)为0.13~1.91,平均值为0.97;多态信息指数(PIC)为0.19~0.81,平均值为0.56;观测杂合度为0.06~0.76,平均值为0.40。聚类分析结果表明,参试的无性系种质材料分为3类,第一类为南林系列无性系;第二类为中林108杨、中潜1号、中潜3-2、中驻2号、中驻4号、中驻6号、中驻7号、中驻8号、浙7、中皖1号、中皖2号、丹红杨、A65/27、南抗4、南抗3;第三类为湘林系列无性系。【结论】TP-M13-SSR分子标记技术能有效地鉴别杨树无性系种质,明晰无性系间亲缘关系,遗传聚类的结果与其谱系关系基本一致。研究结果为杨树无性系种质鉴定及进一步开展杨树杂交育种奠定了基础。
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| 关 键 词： | 杨树 TP-M13-SSR 指纹图谱 聚类分析 |
Construction of SSR fingerprint database of Populus germplasm |
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| LIU Chaofan,ZHANG Guojun,XU Gangbiao.Construction of SSR fingerprint database of Populus germplasm[J].Journal of Central South Forestry University,2021(2). |
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| Authors: | LIU Chaofan ZHANG Guojun XU Gangbiao |
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| Institution: | (College of Forestry,Central South University of Forestry&Technology,Changsha 410004,Hunan,China;Tiger Forest&Paper Group Co.,Ltd,Yueyang 414000,Hunan,China) |
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| Abstract: | 【Objective】The construction of fingerprints data of 141 Populus clones provided theoretical basis for the identification of Populus germplasm and reference for future Populus hybrid breeding.【Method】Eight clones with large genetic background were used for primer screening,141 Populus clones were subjected to SSR-PCR amplification with tailed primer M13 microsatellite markers(TP-M13-SSR),and the PCR amplification products were detected using the automated capillary electrophoresis system ABI3730XL.All sampled clones were genotyped using GeneMarker V1.91,the parameters of polymorphic information conten and the frequency of null alleles were assessed with Cervus 3.0.7.The number of allele,Shannon information index andobserved heterozygosity was carriedout with ARLEQUIN.The clustering analysis was conducted using NTSYS-pc2.1.【Result】A total of 89 polymorphic bands were detected based on13 pairs of SSR primers,and the number of alleles at each locus were 2-12,with an meannumber of 6.5 at one locus.Shannon’s information index(I)ranged from 0.13-1.91,with an mean of 0.97.The polymorphic information index(PIC)ranged from 0.19 to 0.81,with the mean of 0.56.The observed heterozygosity ranged from 0.06 to 0.76,with the mean of 0.40.Cluster analysis performed on all experimental materials could be divided into 3 categories,the first category contains Nanlin clones;the second category contains:‘Zhonglin108’,‘Zhongqian1’,‘Zhongqian 3-2’,‘Zhongzhu 2’,‘Zhongzhu4’,‘Zhongzhu6’,‘Zhongzhu8’,‘Zhe7’,‘Y706’,‘Z450’,‘Danhong’,‘A65/27’,‘S1-8’,‘Nankang 3’,‘ZP80’;the third category includes Xianglin clones.【Conclusion】TP-M13-SSR technology can effectively identify Populus clones and reflect the kinship.The results of this study can provide theoretical basis for the identification of Populus clones and future hybrid breeding work. |
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| Keywords: | Populus TP-M13-SSR fingerprint cluster analysis |
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