Isolation and sequence analysis of a Dof protein gene (PtDof1) in Populus |
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| Authors: | Bei Xin Xiao-yang Chen Kun-xi Ouyang Rui-qi Pian Wei Li |
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| Institution: | (1) Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, 100083, P. R. China;(2) College of Forestry, South China Agricultural University, Guangzhou, 200064, P. R. China |
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| Abstract: | Both cDNA and DNA clones of PtDof1 (GenBank Accession No. FJ402844 and FJ402845) were isolated from plants grown in tissue culture of Populus tomentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading
frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDof1 protein shares 65%, 56%
and 55% identity with Vitis vinifera (CAO48618), Nicotiana tabacum (CAA08755) and Glycine max (ABI16022) Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dof gene family. PtDof1 protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which
may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes. |
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| Keywords: | Populus tomentosa Dof gene family PtDof1 cloning and sequencing |
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