| 两种罗非鱼及其杂交种血清蛋白的聚丙烯酰胺凝胶电泳分离及其雌性特异蛋白氨基酸的分析 |
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| 引用本文： | 刘荣臻,王浩,陈洁平.两种罗非鱼及其杂交种血清蛋白的聚丙烯酰胺凝胶电泳分离及其雌性特异蛋白氨基酸的分析[J].水产学报,1985,9(3):265-273. |
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| 作者姓名： | 刘荣臻 王浩 陈洁平 |
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| 作者单位： | 南京大学生物系
(刘荣臻，王浩)，南京大学生物系(陈洁平) |
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| 摘 要： | 本实验应用聚丙烯酰胺凝胶盘状电泳方法对莫桑比克罗非鱼(Tilapia mossambica)和尼罗罗非鱼(Tilapia milotica)及其杂交种——福寿鱼(T.missambica ?×T.nilotica ?)的血清蛋白进行了分离,同时对其雌性特异蛋白进行了氨基酸组成分析。莫桑比克罗非鱼的血清蛋白电泳图型具有 12—14条蛋白质带,尼罗罗非鱼有12—13条蛋白质带,杂交种—福寿鱼有12—15条蛋白质带;三种鱼血清蛋白电泳图型在雌、雄性别上有明显的差异。莫桑比克罗非鱼和尼罗罗非鱼的成熟雌鱼有两条新的蛋白质带,这些带都比同种的雄鱼所显示的颜色深。杂交种——福寿鱼的血清蛋白电泳图型,为其亲本的中介型。 此外,本文还对雌性特异蛋白的蛋白质带,进行了氨基酸组成分析。
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| 关 键 词： | 杂交种 特异蛋白 尼罗罗非鱼 蛋白质 电泳方法 种鱼 氨基酸组成 血清蛋白电泳 盘状 分离 |
INVESTIGATION ON SEXUAL DIFFERENCE COMPOSITION OF SERUM PROTEIN OF TWO TILAPIA AND THEIR HYBRID |
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| Lin Rongzhen,Wang Hao and Chen Jieping.INVESTIGATION ON SEXUAL DIFFERENCE COMPOSITION OF SERUM PROTEIN OF TWO TILAPIA AND THEIR HYBRID[J].Journal of Fisheries of China,1985,9(3):265-273. |
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| Authors: | Lin Rongzhen Wang Hao and Chen Jieping |
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| Institution: | Department of Biology,University of Nanging;Department of Biology,University of Nanging;Department of Biology,University of Nanging |
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| Abstract: | Serum proteins of Tilapia mossambica. Tilapia nilotica and their hybrid (female T. Mossambica×male T. nilotica) were examined by using 7% polyacrylamide gelelectrophoresis.The T. mossambica serum was isolated into 12--14 protein bands, T. niloticaserum into 12--13 protein bands and the bybrid serum into 13--15 protein bands.Sexual differences in the electrophoretic patterns of serum proteins of the twospecies and their hybrids were compared. The electrophoretic patterns of bandingshowed two new bands in the serum of mature female T. mossombica. They were notobserved in the males. But in the females of T. mossambica and of T. nilotica theprotein bands were broder and darker in colour than those of the males. The eletropho-retic pattern of the hybrids was transient among several components.Various staining methods were used: (a) for total proteins with amide black dyetwo new protein bands appeared; (b) for glycoproteins with the periodic acid-Schiffreaction, the two new protein bands were not visible. However, other protein bandsshowed red colour;(c) for lipoprotein with sudan black B there were two markedprotein bands.For scanning electropherograms on serum of the fish and amins acid compositionof female specific serum protein,ultra-violet-spectro photometer was used. This femalespecific serum protein was characterized by relatively high contents of glycine, pheny-lalanine and amide NH. but the absence of histidine and tyrosine, |
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