| 植物流式细胞遗传学及其应用研究进展* |
| |
| 引用本文： | 李国庆,萧凤回.植物流式细胞遗传学及其应用研究进展*[J].云南农业大学学报,2008,23(3):396-401. |
| |
| 作者姓名： | 李国庆 萧凤回 |
| |
| 作者单位： | 云南农业大学中药材研究所 云南省中药材规范化种植技术指导中心，云南农业大学中药材研究所，云南省中药材规范化种植技术指导中心，云南昆明650201云南农业大学农学与生物技术学院，云南省生物技术创新人才培养基地，云南昆明650201，云南昆明650201云南农业大学农学与生物技术学院，云南省生物技术创新人才培养基地，云南昆明650201 |
| |
| 摘 要： | 流式细胞遗传学是利用流式细胞术对植物有丝分裂细胞的染色体进行分离、纯化和分选等操作的细胞遗传学分支学科，已在许多领域广泛的应用，如与DNA 分子标记结合进行物理作图，利用FISH和PRINS进行细胞遗传作图，重组DNA文库的构建，标记片段的定向分离以及蛋白质分析。利用流式细胞技术已对数十种植物物种进行了染色体分析，应用最多的是豆类作物和禾谷类作物。流式细胞术有许多潜在的利用价值，主要包括植物染色体和染色体臂特异BAC文库的构建，低拷贝序列的目标分离，ESTs和杂交DNA 序列的高产量作图以及对染色体蛋白的整体分析等。本文综述了植物流式细胞遗传学的研究方法及其在植物研究中的应用，并对其在植物基因组分析的潜在利用价值作了展望。
|
| 关 键 词： | 细胞遗传学 流式细胞术 染色体分选 物理作图 |
| 文章编号： | 1004-390X(2008)03-0396-06 |
| 收稿时间： | 2007-07-17 |
| 修稿时间： | 2007年7月17日 |
Advances in Plant Flow Cytogenetics and Its Application |
| |
| LI Guo-qing,XIAO Feng-hui.Advances in Plant Flow Cytogenetics and Its Application[J].Journal of Yunnan Agricultural University,2008,23(3):396-401. |
| |
| Authors: | LI Guo-qing XIAO Feng-hui |
| |
| Institution: | (1．Institute of Chinese Medicinal Materials of Yunnan Agricultural Universit，Yunnan Provincial Center for Chinese Medicinal Material GAP Technology，Kunming 650201，China；2．Faculty of Agronomy and Biotechnology，Yunnan Agricultural University， Center for Agriculture and Biodiversity Research and Training of Yunnan Province，Kunming 650201，China) |
| |
| Abstract: | Flow cytogenetics is one of branches of cytogenetics,involving in the manipulations such as isolation,purification and sorting of chromosomes from mitosis cells by flow cytometry.Cytogenetics has been applied widely in many fields,including physical mapping by PCR,high-resolution cytogenetic mapping by FISH and PRINS,construction of recombinant DNA libraries,targeted isolation of marker fragment and analysis of proteins.Flow cytometry has been used for the analysis and sorting of chromosome in dozens of plant species and applied most in legume and cereal crops.A great potential of flow cytometry is foreseen in construction of chromosome and chromosome-arm-specific BAC libraries,targeted isolation of low-copy sequences,high-throughput physical mapping of ESTs and other DNA sequences by hybridization to DNA arrays,and global characterization of chromosomal proteins.The methodology and application of flow cytogenetics in plant were reviewed in this article. |
| |
| Keywords: | plant flow cytogenetics flow cytometry chromosome sorting physical mapping |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《云南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《云南农业大学学报》下载免费的PDF全文 |
