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| 牛奶及奶粉中三聚氰胺残留酶联免疫检测方法的研究 | |
| 引用本文： | 张建勋,王战辉,王亚辉,吴小平,李建成,王照鹏,江海洋,沈建忠.牛奶及奶粉中三聚氰胺残留酶联免疫检测方法的研究[J].中国兽医杂志,2012,48(7):74-76. |
| 作者姓名： | 张建勋 王战辉 王亚辉 吴小平 李建成 王照鹏 江海洋 沈建忠 |
| 作者单位： | 1. 中国农业大学动物医学院,北京海淀100193;辽宁省畜牧兽医局,辽宁沈阳110001 2. 中国农业大学动物医学院,北京海淀,100193 3. 北京维德维康生物技术有限公司,北京海淀,100085 |
| 基金项目： | "十一五"国家科技支撑计划课题，教育部长江学者和创新团队发展计划 |
| 摘 要： | 通过4,6-二氨基-2-氯-1,3,5-三嗪与对氨基苯丁酸反应获得三聚氰胺半抗原,再以活性酯法与载体蛋白偶联制备三聚氰胺免疫原或包被原。免疫BALB/c小鼠,利用杂交瘤技术制备出针对三聚氰胺的特异性单克隆抗体。采用间接竞争酶联免疫吸附法(ciELISA)建立检测三聚氰胺的标准曲线,线性范围是17.4～345.5ng/mL,50%抑制浓度(IC50)61.3ng/mL。牛奶和奶粉加标回收率在68.1%～91.0%,变异系数在2.4%～14.5%。结果表明,该方法可以满足牛奶和奶粉中三聚氰胺残留分析要求。 |
| 关 键 词： | 牛奶 奶粉 三聚氰胺 ciELISA |
Development of an Enzyme Linked Immunosorbent Assay for Detection of Melamine Residues in Milk and Milk Powder |
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| ZHANG Jian-xun , WANG Zhan-hui , WANG Ya-hui , WU Xiao-ping , LI Jian-cheng , WANG Zhao-peng , JIANG Hai-yang , SHEN Jian-zhong.Development of an Enzyme Linked Immunosorbent Assay for Detection of Melamine Residues in Milk and Milk Powder[J].Chinese Journal of Veterinary Medicine,2012,48(7):74-76. | |
| Authors: | ZHANG Jian-xun WANG Zhan-hui WANG Ya-hui WU Xiao-ping LI Jian-cheng WANG Zhao-peng JIANG Hai-yang SHEN Jian-zhong |
| Institution: | 1(1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China; 2.Bureau of Animal Health and Production of Liaoning Province,Shenyang 110001,China; 3.Beijing WDWK Biotechnology Company,Ltd.,Beijing 100085,China) |
| Abstract: | 4-aminobenzenebutanoic acid was reacted with 2-chloro-4,6-diamino-1,3,5-triazine to get hapten of melamine.An immunogen or coating antigen was prepared by coupling hapten to carrier protein with the activated ester method.BALB/c mice were immunized and the specific monoclonal antibody against melamine was generated with hybridoma technique.In a competitive indirect enzyme-linked immunosorbent assay(ciELISA),the linear range of calibration curve to detect melamine was 17.4 ng/mL~345.5 ng/mL with an 50% binding inhibition(IC50) value of 61.3 ng/mL.Recoveries ranged from 68.1% to 91.0% for fortified milk and milk powder at various levels with coefficients of variation between 2.4%~14.5%.The results indicated that the method for the detection of melamine residues in milk and milk powder was effective. |
| Keywords: | milk milk powder melamine ciELISA |
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