| 15N同位素稀释法测定燕麦根际固氮菌固氮量的研究 |
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| 引用本文： | 席琳乔,张德罡,姚拓.15N同位素稀释法测定燕麦根际固氮菌固氮量的研究[J].核农学报,2007,21(4):417-420. |
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| 作者姓名： | 席琳乔 张德罡 姚拓 |
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| 作者单位： | 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆,阿拉尔市,843300;新疆生产建设兵团塔里木畜牧科技重点实验室,新疆,阿拉尔市,843300;甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省科技攻关项目
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甘肃省教育厅科研项目
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新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放项目 |
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| 摘 要： | 用15N同位素稀释法对接种到燕麦上的不同固氮菌的固氮能力进行了测定,结果表明:固氮菌处理的地上植株15N原子百分超为1.0871%～1.3791%,其中Pseudomonas spN4最低(1.0871%);植株地下部分为1.0921%～1.2751%,Pseudomonas spN4也是最低(1.0921%)。Pseudomonas spN4、A.lipoferumO6和Zoogloea spW6固氮能力较好。植株全氮含量增加2.08%～39.58%,A.lipoferumO6处理的全氮含量最高(39.58%)。接种固氮菌能够提高燕麦的全氮含量、固氮百分率和固氮量。
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| 关 键 词： | 燕麦 固氮菌 固氮量 15N同位素稀释 |
| 文章编号： | 1000-8551（2007）04-417-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2006-11-15 |
UING ~(15)N ISOTOPIC DILUTION METHOD TO DETERMINE THE QUANTIFICATION OF ASSOCIATION NITROGEN FIXATION BACTERIA SOIL FROM OAT ROOTS |
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| XI Lin-qiao,ZHANG De-gang,YAO Tuo.UING ~(15)N ISOTOPIC DILUTION METHOD TO DETERMINE THE QUANTIFICATION OF ASSOCIATION NITROGEN FIXATION BACTERIA SOIL FROM OAT ROOTS[J].Acta Agriculturae Nucleatae Sinica,2007,21(4):417-420. |
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| Authors: | XI Lin-qiao ZHANG De-gang YAO Tuo |
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| Abstract: | The quantification of nitrogen fixed by associative nitrogen fixing bacteria,which separated from oat and wheat were re-inoculated to oat,was analyzed using (~(15)N) isotopic dilution technique.Results showed that most strains were beneficial to nitrogen fixation.(~(15)N) atom% excesses in the above ground part of plant ranged from 1.0871% to 1.3791%,and in underground part were from 1.0921% to 1.2751%.The lowest ~(15)N atom% excess found in Pseudomonas sp N4 treatment was 1.0871% and 1.0921% in the above and under ground parts of oat plant respectively.Pseudomonas sp N4,A.lipoferum O6 and Zoogloea sp.W6 had higher ability of nitrogen fixation.Nitrogen content in oat plant was increased form 2.08% to 39.58%,of which the highest content of total Nitrogen was detected in A.lipoferum O6.Inoculation of nitrogen fixing bacteria could increase total nitrogen content,nitrogen fixing percent and amount of fixed nitrogen. |
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| Keywords: | oat(Avina sativa L ) associative nitrogen fixing bacteria the quantification of biological nitrogen fixed ~(15)N isotopic dilution technique |
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