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| 小麦SSCP分子标记体系的优化 | |
| 引用本文： | 余桂红,唐克轩,马鸿翔,周淼平,张旭,任丽娟,陆维忠.小麦SSCP分子标记体系的优化[J].核农学报,2007,21(4):333-338. |
| 作者姓名： | 余桂红 唐克轩 马鸿翔 周淼平 张旭 任丽娟 陆维忠 |
| 作者单位： | 1. 上海交通大学复旦-交大-诺丁汉植物生物技术研发中心,上海,200030;江苏省农业科学院农业生物技术研究所,江苏,南京,210014 2. 上海交通大学复旦-交大-诺丁汉植物生物技术研发中心,上海,200030 3. 江苏省农业科学院农业生物技术研究所,江苏,南京,210014 |
| 摘 要： | SSCP技术易受上样缓冲液、变性的时间及温度、电泳条件等诸多因素的影响。本研究对上样缓冲液、变性的时间及温度、电泳条件等影响因素进行了研究,结果显示:上样缓冲液中不添加甘油、用量为PCR产物体积的1.5～3倍、变性温度98℃、变性时间10～15min、电泳缓冲液为0.5×TBE,常温下恒功率80W(2.67W/cm)电泳2 h左右,单链DNA条带清晰,易于识别。 |
| 关 键 词： | 小麦 SSCP 优化 |
| 文章编号： | 1000-8551（2007）04-333-06 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2006-12-08 |
OPTIMIZATION OF THE CONDITIONS AFFECTING SSCP TECHNIQUE IN WHEAT |
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| YU Gui-hong,TANG Ke-xuan,MA Hong-xiang,ZHOU Miao-ping,ZHANG Xu,REN Li-jian,LU Wei-zhong.OPTIMIZATION OF THE CONDITIONS AFFECTING SSCP TECHNIQUE IN WHEAT[J].Acta Agriculturae Nucleatae Sinica,2007,21(4):333-338. | |
| Authors: | YU Gui-hong TANG Ke-xuan MA Hong-xiang ZHOU Miao-ping ZHANG Xu REN Li-jian LU Wei-zhong |
| Abstract: | Single Strand Conformation Ploymorphism(SSCP) technique is easy to be affected by a good many factors such as loading buffer,denaturalization temperature and time,and electrophoresis factors.The influences of loading buffer,denaturalization temperature and time and electrophoresis factors(electrophoresis temperature,electrophoresis buffer and electrophoresis power) on SSCP technique were studied in wheat.The results were as follows when volume of the loading buffer without glycerol was 1.5～3 times of the volume of the PCR buffer;denaturalization temperature was 98℃;denaturalization time was 10～15 minutes;electrophoresis buffer was 0.5×TBE;the electrophoresis temperature was about 25℃;the electrophoresis power was 80W(2.67W/ cm),the bands of the Single Strand DNA was clear and easy to read. |
| Keywords: | wheat SSCP optimization |
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