| 内生枯草芽孢杆菌B-001菌株内生定殖研究及生物学特性 |
| |
| 引用本文： | 易有金,罗坤,罗宽,刘二明.内生枯草芽孢杆菌B-001菌株内生定殖研究及生物学特性[J].核农学报,2007,21(4):349-352. |
| |
| 作者姓名： | 易有金 罗坤 罗宽 刘二明 |
| |
| 作者单位： | 1. 湖南农业大学生物安全科技学院,湖南,长沙,410128
2. 广西大学农学院,广西,南宁,530005 |
| |
| 摘 要： | 用浸种、浸根、淋根、伤茎、伤叶和喷雾等接种方法测试枯草芽孢杆菌B-001菌株入侵烟草植株的途径,发现该菌可通过自然孔口和伤口入侵寄主植物;取接种植株的茎部组织切片在电镜下观察,发现该菌株在烟草的微管束和细胞内定殖。生长特性研究结果表明该菌株最适生长pH为7.5,最适生长温度为30℃,48～96h内处于稳定生长期。
|
| 关 键 词： | 烟草 内生枯草芽孢杆菌B-001 内生定殖 生物学特性 |
| 文章编号： | 1000-8551（2007）04-349-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2006-12-03 |
ENDOPHYTE B-001(Bacillus subtilis) COLONIZATION AND ITS BIOLOGICAL CHARACTERS |
| |
| YI You-jin,LUO Kun,LUO Kuan,LIU Er-ming.ENDOPHYTE B-001(Bacillus subtilis) COLONIZATION AND ITS BIOLOGICAL CHARACTERS[J].Acta Agriculturae Nucleatae Sinica,2007,21(4):349-352. |
| |
| Authors: | YI You-jin LUO Kun LUO Kuan LIU Er-ming |
| |
| Institution: | 1. College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128;2. Agricultural College, Guangxi University, Nanning , Guangxi , 530005 |
| |
| Abstract: | The different inoculation methods,such as dipping seed,dipping root,watering root,injecting stem,injecting leaf,praying were used to test how the endophyte B-001(Bacillus subtilis) entered the host plant.The results indicated that the bacteria penetrated into plant tissues through natural wounds.Observing the plant tissue after inoculation 30 days by electron microscope,strain B001 could be found in vascular and cell.Study on its biological character showed that the optimal pH and temperature for the growth of B-001 were 7.5 and 30℃,respectively,and its stable growth stage was 48～96h. |
| |
| Keywords: | tobacco endophyte Bacillus subtilis B-001 endophytice colonization biological characters |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
