|
|||||
|
|
| 艳丽花楸组培快繁技术研究 | |
| 引用本文： | 陈士刚,陶晶,秦彩云,李青梅,侯星晔,梁泽平.艳丽花楸组培快繁技术研究[J].吉林林业科技,2010,39(5):12-15. |
| 作者姓名： | 陈士刚 陶晶 秦彩云 李青梅 侯星晔 梁泽平 |
| 作者单位： | 1. 吉林省林业科学研究院,吉林,长春,130033 2. 吉林市松花湖风景区园林管理处,吉林,吉林,132013 3. 吉林省汪清林业局,吉林,延边,133200 |
| 摘 要： | 以艳丽花楸的侧芽和种子萌发苗为外植体,MS为基本培养基,添加不同配比的植物生长调节剂,进行离体培养。结果表明:较之种子萌发苗,当年侧芽外植体更适合作为诱导材料。在芽的诱导及增殖阶段,当激素水平为6-BA0.5 mg.L-1+NAA0.10 mg.L-1时,增殖效果较好,分化率可达90%,单芽平均分化芽数大于5个。在生根诱导阶段,采用质量浓度为2.8 mg.L-1的IBA较为合适,生根率可达到95%。 |
| 关 键 词： | 艳丽花楸 组织培养 快繁技术 |
Tissue culture and rapid propagation of Sorbus decora |
|
| CHEN Shi-gang,TAO Jing,QIN Cai-yun,LI Qing-mei,HOU Xing-ye,LIANG Ze-ping.Tissue culture and rapid propagation of Sorbus decora[J].Jilin Forestry Scicnce and Technology,2010,39(5):12-15. | |
| Authors: | CHEN Shi-gang TAO Jing QIN Cai-yun LI Qing-mei HOU Xing-ye LIANG Ze-ping |
| Institution: | 1.Jilin Provincial Academy of Forestry Sciences,Changchun 130033,China;2.Garden Administration Office of Sohua Lake in Jilin City,Jilin 132013,China;3.Wangqing Forestry Bureau of Jilin Provice,Yanbian 133200,China) |
| Abstract: | Lateral buds and seedlings of Sorbus decora were cultured for callus induction on MS medium supplemented with different hormone combinations.The results showed that the 1-yr-old lateral bud explants was a more suitable material than seedlings.The optimal hormone concentration for the proliferation and propagation was 6-BA0.5 mg·L-1＋NAA0.10 mg·L-1,and the differentiation rate reached to 90%,with more than 5 buds differentiated from single bud on average.The optimal rooting medium was 2.8 mg·L-1of IBA,and the rooting rate reached to 95%. |
| Keywords: | Sorbus decora tissue culture rapid propagation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |