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| 超低温冷冻对小鼠桑椹胚多能性因子Oct-4表达的影响 | |
| 引用本文： | 周光斌,郭将,曾艳,程柯仁,傅祥伟,朱士恩.超低温冷冻对小鼠桑椹胚多能性因子Oct-4表达的影响[J].中国畜牧杂志,2013,49(9). |
| 作者姓名： | 周光斌 郭将 曾艳 程柯仁 傅祥伟 朱士恩 |
| 作者单位： | 1. 四川农业大学动物科技学院动物遗传育种研究所,四川成都 611130;中国农业大学动物科技学院,北京 100193 2. 四川农业大学动物科技学院动物遗传育种研究所,四川成都,611130 3. 中国农业大学动物科技学院,北京,100193 |
| 基金项目： | 四川农业大学"双支计划"，大学生科研兴趣培养计划 |
| 摘 要： | 本实验旨在探讨昆明白小鼠桑椹胚超低温冷冻后Oct-4表达的变化与胚胎发育潜力的关系。胚胎采用OPS法冷冻,即胚胎于10%EG+10%DMSO溶液中预处理30 s,然后再移入玻璃化溶液(EDFS30)中处理25 s,以OPS为承载器投入液氮中。毒性组胚胎未投入液氮,其他过程与冷冻组相同,新鲜胚胎为对照组。胚胎解冻后,检测Oct-4 mRNA与蛋白的表达,通过观察其形态正常率、囊胚发育率和囊胚细胞数来综合判断其体外发育能力。结果表明:冷冻组和毒性组较桑椹胚中Oct-4 mRNA的表达量对照组相比显著降低(P<0.05),蛋白表达量3组间无显著差异。桑椹胚处理后的形态正常率以及桑椹胚培养48 h后的囊胚发育率(96.67%～100%)和囊胚细胞数(89.67～92.33)3组间无显著差异。结果显示,玻璃化冷冻保存小鼠桑椹胚后多能性基因Oct-4 mRNA表达的变化并未影响其体外发育潜力。 |
| 关 键 词： | Oct-4 超低温冷冻 桑椹胚 昆明白小鼠 |
Oct-4 Expression in Mouse Morula after Cryopreservation |
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| ZHOU Guang-bin , GUO Jiang , ZENG Yan , CHENG Ke-ren , FU Xiang-wei , ZHU Shi-en.Oct-4 Expression in Mouse Morula after Cryopreservation[J].Chinese Journal of Animal Science,2013,49(9). | |
| Authors: | ZHOU Guang-bin GUO Jiang ZENG Yan CHENG Ke-ren FU Xiang-wei ZHU Shi-en |
| Abstract: | This study was conducted to investigate the effect of cryopreservaion on oct-4 expression and the in vitro development of Kunming mouse morula.The morulas were randomly allocated into three groups: untreated(control),exposed to vitrification solution without being plunged into liquid nitrogen(toxicity),or vitrified by open-pulled straw method(vitrification).After vitrification and warming,the embryos were morphologically evaluated and assessed by their development to blastocysts,or by the average cell number of blastocyst.Oct-4 expression in morula was assessed by qRT-PCR and western blotting.The results showed that the oct-4 mRNA in toxicity and vitrification groups were significantly lower(P<0.05) than that of control.However,there was no significant difference in oct-4 expression at protein level among three groups.The blastocyst rate(96.67% to 100.00%) and the average cell number of blastocyst(89.67 to 92.33) were similar among them.The data demonstrated that the decreased oct-4 mRNA due to cryopreservation didn'tnegatively affect the in vitro developmental potential of mouse morula. |
| Keywords: | oct-4 cryopreservation morula Kunming white mous |
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