| 低氮胁迫下拟南芥低氮耐性突变体lnt1的氮代谢特征 |
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| 作者姓名： | TENG Yi-Bo LI Ya-Juan FANG Ping LA Gui-Xiao |
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| 作者单位： | TENG Yi-Bo,LA Gui-Xiao(Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029,China);LI Ya-Juan(Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutrition,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029,China);FANG Ping(Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029,China;Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutri) |
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| 基金项目： | *1 National Basic Research Program of China (No.2007CB109305) and the National Natural Science Foundation of China (No.30370839). |
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| 摘 要： | A growth experiment on agar medium and a hydroponics experiment were carried out to study the nitrogen (N) metabolism of a low-N tolerant mutant (lnt1) of Arabidopsis thaliana under different N levels as compared with the wildtype (WT) Arabidopsis. On the agar medium, no apparent growth differences were observed between the lnt1 and WT plants under a normal N level of 9 mmol L-1 NO3-. However, under a low N level of 0. 18 mmol L-1 NO3-, the growth of the WT plants was greatly retarded, while the lnt1 plants were not affected by low-N stress and showed similar growth with those grown under a normal N level. In the hydroponics experiment, the lnt1 mutant had higher activities of glutamine synthetase (GS) and NADH-dependent glutamate synthase (NADH-GOGAT) in both leaves and roots under N-deficient conditions. Moreover, they accumulated less ammonium (NH4+) but more free amino acids in leaves compared with the WT plants. These observations suggest that better N assimilation might contribute to the low-N tolerant phenotype of the lnt1 mutant.
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| 关 键 词： | ammonium free amino acids glutamate synthase glutamine synthetase nitrogen assimilation |
| 收稿时间： | 28 February 2010 |
Characterization of nitrogen metabolism in the low-nitrogen tolerant lnt1 mutant of Arabidopsis thaliana under nitrogen stress |
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| TENG Yi-Bo,LI Ya-Juan,FANG Ping,LA Gui-Xiao.Characterization of nitrogen metabolism in the low-nitrogen tolerant lnt1 mutant of Arabidopsis thaliana under nitrogen stress[J].Pedosphere,2010,20(5):623-632. |
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| Authors: | TENG Yi-Bo LI Ya-Juan FANG Ping and LA Gui-Xiao |
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| Institution: | Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029 (China)
;Zhejiang Key Laboratory of Subtropical Soil Science and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029 (China);Zhejiang Key Laboratory of Subtropical Soil Science and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029 (China);Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029 (China)
;Zhejiang Key Laboratory of Subtropical Soil Science and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029 (China);Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029 (China) |
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| Abstract: | A growth experiment on agar medium and a hydroponics experiment were carried out to study the nitrogen (N) metabolism of a low-N tolerant mutant (lnt1) of Arabidopsis thaliana under different N levels as compared with the wild-type (WT) Arabidopsis. On the agar medium, no apparent growth differences were observed between the lnt1 and WT plants under a normal N level of 9 mmol L-1 NO3-. However, under a low N level of 0.18 mmol L-1 NO3-, the growth of the WT plants was greatly retarded, while the lnt1 plants were not affected by low-N stress and showed similar growth with those grown under a normal N level. In the hydroponics experiment, the lnt1 mutant had higher activities of glutamine synthetase (GS) and NADH-dependent glutamate synthase (NADH-GOGAT) in both leaves and roots under N-deficient conditions. Moreover, they accumulated less ammonium (NH4+) but more free amino acids in leaves compared with the WT plants. These observations suggest that better N assimilation might contribute to the low-N tolerant phenotype of the lnt1 mutant. |
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| Keywords: | ammonium free amino acids glutamate synthase glutamine synthetase nitrogen assimilation |
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