| 影响黑果枸杞缩合单宁测定的因素分析 |
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| 引用本文： | 房江育,马雪泷.影响黑果枸杞缩合单宁测定的因素分析[J].中国农学通报,2006,22(8):105-105. |
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| 作者姓名： | 房江育 马雪泷 |
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| 作者单位： | 1. 黄山学院生物系,安徽,黄山,245021;农业部安徽农业大学茶叶生物化学与生物技术重点实验室,安徽,合肥,360023
2. 黄山学院生物系,安徽,黄山,245021 |
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| 基金项目： | 安徽省教育厅“安徽省高等学校‘十五’优秀人才计划——安徽省高校中青年学科带头人培养对象”资助。 |
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| 摘 要： | 为了探讨黑果枸杞中缩合单宁的测定，采用方差分析方法研究了干燥方法、粉碎度以及提取溶剂对黑果枸杞枝叶中缩合单宁提取量的影响。结果表明各因素中主效与互作同时存在，尤其是干燥方法与提取液之间、提取液与粉碎度之间的搭配会影响最终的缩合单宁提取量。冷冻干燥、粉碎后过80~100目筛以及用甲醇酸水溶液提取能够获得最大的提取量。
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| 关 键 词： | 日光温室 日光温室 优势 问题 对策 |
| 收稿时间： | 2006-04-13 |
| 修稿时间： | 2006-04-132006-04-18 |
Factors Influencing the Assay of Condensed Tannin in Lycium Ruthenicum Murr. |
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| Fang Jiangyu,Ma Xuelong.Factors Influencing the Assay of Condensed Tannin in Lycium Ruthenicum Murr.[J].Chinese Agricultural Science Bulletin,2006,22(8):105-105. |
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| Authors: | Fang Jiangyu Ma Xuelong |
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| Institution: | 1.Biology Department of Huangshan University, Huangshan 245021; 2.Tea Biochemistry and Biotechnology Laboratory of Ministry of Agriculture, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | Factors including dry method, comminution and extracting effect on the extraction amount of condensed tannin in the leaf of Lycium ruthenicum Murr were studied. To get the detail relationship among these parameters, different level of those factors was set when measuring the condensed tannin, and the results were analyzed by statistical methods. The results show that both the main effect of a single factor and the mutual action of the factors existed, especially the action between the dry method and the extract, and the action between the commimution and the extract. Freezing dry was better than air dry, medium size particle was better than the large and small particles and the acidic methyl alcohol solution was better than that without acid. Of all the treatments, the combination of freezing dry, 80~100 mesh sieve and 1% HCl in 70% methyl alcohol could yield the largest amount of condensed tannin from the leaf of Lycium ruthenicum Murr. |
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| Keywords: | Condensed tannin Lycium ruthenicum Murr Assay |
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