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| 甘蓝型黄籽油菜分子标记辅助选择的效果分析 | |
| 引用本文： | 刘志文,王英,刘雪平,傅廷栋,薛永常,涂金星,马朝芝.甘蓝型黄籽油菜分子标记辅助选择的效果分析[J].华北农学报,2006,21(2):57-61. |
| 作者姓名： | 刘志文 王英 刘雪平 傅廷栋 薛永常 涂金星 马朝芝 |
| 作者单位： | 1. 大连轻工业学院生物与食品工程学院,辽宁,大连,116034;华中农业大学,作物遗传改良国家重点实验室,湖北,武汉,430070 2. 大连轻工业学院生物与食品工程学院,辽宁,大连,116034 3. 华中农业大学,作物遗传改良国家重点实验室,湖北,武汉,430070 |
| 基金项目： | 国家“973”项目(2001CB108) 辽宁省教育厅项目(05L075) |
| 摘 要： | 应用分子标记辅助选择的方法,将黄籽性状导入到波里马恢复系中,利用杂种优势,将是提高油菜产量的理想途径。分析了分子标记辅助选择甘蓝型黄籽油菜的前景和背景选择的效果,结果表明,在背景选择中,7种不同 RAPD引物数目组合方式的选择结果基本一致;应用少数连锁群上的标记可以获得与全基因组相似的选择结果。在前景选择中,随着世代的增加,每一个标记的选择准确率在降低;双侧标记的选择准确率比单侧的标记高,此外,多个单侧标记未能提高选择准确率。据此,提出了作物前景和背景选择过程中的一些改进策略。 |
| 关 键 词： | 甘蓝型黄籽油菜 分子标记 前景选择 背景选择 |
| 文章编号： | 1000-7091（2006）02-0057-05 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005-09-28 |
The Effects Analysis of Molecular Marter-assisted Selection in Yellow-seeded Brassica napus |
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| LIU Zhi-wen,WANG Ying,LIU Xue-ping,FU Ting-dong,XUE Yong-chang,TU Jin-xing,MA Chao-zhi.The Effects Analysis of Molecular Marter-assisted Selection in Yellow-seeded Brassica napus[J].Acta Agriculturae Boreali-Sinica,2006,21(2):57-61. | |
| Authors: | LIU Zhi-wen WANG Ying LIU Xue-ping FU Ting-dong XUE Yong-chang TU Jin-xing MA Chao-zhi |
| Abstract: | To transfer the yellow-seeded gene into Pol cms restorer lines and utilize heterosis using molecular marterassisted selection (MAS) would be an ideal approach to increase the rapeseed oil yield. This paper analyzed the effects of the back and foreground selection in yellow-seeded Brassica napus , the results showed that the selection results of 7 different RAPD primer combinations are identical and selection by a limited linkage groups can get the whole genome similar effect in background selection. During foreground selection program, it demonstrated that the selection efficiency of each marker would decrease with generation and the unilateral markers can't increase the efficiency with number, moreover, flanked markers can attain higher efficiency than unilateral markers. As a result, we proposed some improved strategies for back and foreground selection in crop MAS. |
| Keywords: | Yellow-seeded Brassica napus Molecular marker Background selection Foreground selection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |