| 磺酸化丝素蛋白的二级结构及免疫原性研究 |
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| 引用本文： | 李绍群,颜辉,唐玉斌,陈新军,段艳波.磺酸化丝素蛋白的二级结构及免疫原性研究[J].蚕业科学,2013(1):88-94. |
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| 作者姓名： | 李绍群 颜辉 唐玉斌 陈新军 段艳波 |
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| 作者单位： | 江苏科技大学生物与化学工程学院;镇江市第一人民医院骨科 |
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| 基金项目： | 江苏省普通高校研究生科研创新计划项目(No.CXZZ12_0715) |
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| 摘 要： | 以丝素蛋白为原料,采用偶氮盐法进行修饰获得磺酸化丝素蛋白。利用衰减全反射傅里叶变换红外光谱(ATR-FT-IR)仪获取磺酸化丝素蛋白及不同有机溶剂处理后的样品光谱,通过傅里叶自退卷积、二阶导数及曲线拟合方法测算磺酸化丝素蛋白各二级结构比率的变化,并对其免疫原性进行动物试验分析,探讨磺酸化丝素蛋白在医学组织工程的应用价值。冷冻干燥得到的磺酸化丝素蛋白二级结构β-折叠、无规卷曲、α-型、β-转角的比率依次为10.97%±1.32%、59.92%±2.72%、17.50%±2.13%、11.61%±1.17%。磺酸化丝素蛋白经不同种类有机醇溶剂处理后,诱导二级结构变化的速度不同,并随着处理时间的延长,诱导磺酸化丝素蛋白结构改变的速率减缓。经有机醇溶剂处理后,二级结构中的无规卷曲转变成SilkⅠ型的α-型,然后转变成较稳定的SilkⅡ型的β-折叠,且在不同溶剂中无规卷曲和α-型间可相互转化。通过小鼠脾细胞(T、B细胞)的刺激反应性试验发现磺酸化丝素蛋白的免疫原性低。研究结果表明,磺酸化丝素蛋白具有较低免疫原性以及其二级结构可以通过有机醇类进行调节的特点,有望开发成为一种生物相容性优良、可降解吸收的组织工程及药物缓控释材料。
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| 关 键 词： | 丝素蛋白 修饰 磺酸化 二级结构 免疫原性 |
Study on the Secondary Structure and Immunogenicity of Sulfonated Silk Fibroin |
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| LI Shao-Qun,YAN Hui, TANG Yu-Bin,CHEN Xin-Jun,DUAN Yan-Bo.Study on the Secondary Structure and Immunogenicity of Sulfonated Silk Fibroin[J].Acta Sericologica Sinica,2013(1):88-94. |
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| Authors: | LI Shao-Qun YAN Hui TANG Yu-Bin CHEN Xin-Jun DUAN Yan-Bo |
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| Institution: | 1(1School of Biological and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2Department of Orthopedics,Zhenjiang First People’s Hospital,Zhenjiang Jiangsu 212002,China) |
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| Abstract: | Silk fibroin was used as the material for preparing sulfonated silk fibroin(SSF) by means of diazonium salt coupling.The spectrum of SSF treated with different organic solvents was obtained by attenuated total reflectance-Fourier transformed infrared spectrum(ATR-FTIR).The proportional variation of various SSF secondary structures was assessed through Fourier self-deconvolution(FSD),second derivative and curve fitting.Moreover,animal test was conducted to analyze its immunogenicity so as to explore practical value of SSF in medical tissue engineering.The proportion of β-sheet,random coil,α-form and β-turn in lyophilized SSF was found to be 10.97%±1.32%,59.92%±2.72%,17.50%±2.13%,and 11.61%±1.17%,respectively.After being treated with different organic alcohol solutions,the SSF had various rates in secondary structure change which showed a slow-down trend with treatment time.After organic alcohol treatment,the random-coil secondary structure was transformed into α-form of Silk I type and then transformed into more stable β-sheet of Silk II type.Nevertheless,random coil and α-form could be converted into each other in different solvents.Stimulatory reaction test in mouse spleen cells(T-and B-cells) revealed that SSF has low immunogenicity.The results show that SSF has low immunogenicity and its secondary structure is adjustable by organic alcohol,being promising for development of tissue engineering material with excellent biocompatibility and biodegradablility for slow and controlled release of drug. |
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| Keywords: | Silk fibroin Modification Sulfonation Secondary structure Immunogenicity |
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