Isolation,Characterization and Quantification of Tricin and Flavonolignans in the Medicinal Rice Njavara (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.), as Compared to Staple Varieties |
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| Authors: | Smitha?Mohanlal Rathnam?Parvathy Vasantha?Shalini Antony?Helen Email author" target="_blank">Ananthasankaran?JayalekshmyEmail author |
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| Institution: | (1) Chemical Sciences & Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate P.O., Thiruvananthapuram, 695019, Kerala, India;(2) Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram, 695 581, India |
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| Abstract: | Njavara is an important medicinal rice variety of Kerala, India, widely used in Ayurveda as a ‘health food’ and in the treatment
of rheumatoid arthritis, paralysis, neurodegenerative diseases and in rejuvenation therapy. Phytochemical investigations and
spectroscopic studies of the diethyl ether fraction of methanolic extract of Njavara Black (NB) rice bran gave three important
compounds namely, tricin and two rare flavonolignans- tricin 4′-O-(erythro-β-guaiacylglyceryl) ether and tricin 4′-O-(threo-β-guaiacylglyceryl) ether. The EC50 values of these compounds in DPPH system were 90.39, 352.04 and 208.1 μg/ml, respectively. Quantification of the compounds
by HPLC in NB and staple, non-medicinal rice varieties Sujatha (SJ) and Palakkadan Matta (PM) showed that tricin is present
39.64 and 16.12 fold higher in NB, compared to SJ and PM, respectively. This is the first report on the occurrence of tricin
at significantly higher levels in Njavara and occurrence of the two flavonolignans in Oryza sativa species. Of the three compounds, tricin and the threo- form of flavonolignan showed anti-inflammatory effect of >65% after 5 h, at 2 mg/kg, in carrageenan-induced, paw edema experiments
in rats. The results of the study corroborate with the preferential use of Njavara in indigenous medicine, over staple varieties. |
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