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| 比较3种间接ELISA试验检测口蹄疫感染抗体的研究 | |
| 引用本文： | 龚振华,徐宝娟,范根成,胡永浩,陈书琨,董自珍,王若聪,祁会彩,蒋正军,郑增忍.比较3种间接ELISA试验检测口蹄疫感染抗体的研究[J].中国动物检疫,2005,22(12):27-29. |
| 作者姓名： | 龚振华 徐宝娟 范根成 胡永浩 陈书琨 董自珍 王若聪 祁会彩 蒋正军 郑增忍 |
| 作者单位： | 1. 农业部动物检疫所,青岛,266032 2. 甘肃农业大学 3. 深圳质量技术监督检验检疫局 4. 天津质量技术监督检验检疫局 |
| 摘 要： | 将3种检测口蹄疫非结构蛋白抗体的间接ELISA试验进行了比较。这3种间接ELISA试验检测田间血清样品的最低符合率为96％，最高符合率达98％：有2种间接ELISA试验可以检测到感染口蹄疫第10天后血清中的口蹄疫感染抗体．有1种间接ELISA试验可以检测到感染口蹄疫第14天后血清中的口蹄疫感染抗体；这3种间接ELISA试验检测12份已知口蹄疫阳性血清的试验结果吻合。研究表明．这3种间接ELISA试验方法对于检测口蹄疫感染抗体具有较好的特异性．其中猪口蹄疫3A蛋白间接ELISA诊断试剂盒在灵敏性、特异性和符合率方面更优于另外2种试剂盒。 |
| 关 键 词： | 口蹄疫 非结构蛋白 ELISA 田间血清样品 |
| 文章编号： | 1005-944X(2005)12-0027-03 |
Studies on Comparison of 3 Indirect ELISAs for Detection of Foot and Mouth Disease Infection Antibody |
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| Gong Zhenhu,Xu Baojuan,Fan Gencheng,Hu Yonghao,Chen Shukun Dong Zizhen,Wang Ruocong,Qiu Huicai,Jiang Zhengjun,Zheng Zengren.Studies on Comparison of 3 Indirect ELISAs for Detection of Foot and Mouth Disease Infection Antibody[J].China Journal Of Animal Quarantine,2005,22(12):27-29. | |
| Authors: | Gong Zhenhu Xu Baojuan Fan Gencheng Hu Yonghao Chen Shukun Dong Zizhen Wang Ruocong Qiu Huicai Jiang Zhengjun Zheng Zengren |
| Institution: | National Animal Quarantine institute,MOA.Qingdao 266032 |
| Abstract: | It is found in camparing the 3 Indirect ELISAs for that the least coincidence of the three methods in testing field detection of non-structune protein antibcdies serum samples were 96% and the maximal coincidence was 98%; WO indirect ELISAs could detect FMD antibody in serum 10 days after infection,the other detected FMD antibody 14 days offer infection.All the 3 indirect ELISAs showed positive reactions to the 12 known FMD positine serum samples.Studies indicated that all the 3 indirect ELISAs are specific for detection of FMD infection antibody and the indirect 3A protein ELISA is better than the other 2 ELISA kits in specificity.sensitivity and coincidence. |
| Keywords: | Foot and mouth disease non-structure protein ELISA |
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