| 樟树叶蛋白提取工艺及四季含量动态分析 |
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| 引用本文： | 王娜,褚衍亮,李静,王凯旋.樟树叶蛋白提取工艺及四季含量动态分析[J].北方园艺,2011(7):51-54. |
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| 作者姓名： | 王娜 褚衍亮 李静 王凯旋 |
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| 作者单位： | 江苏科技大学,生物与环境工程学院,江苏,镇江,212018;江苏科技大学,生物与环境工程学院,江苏,镇江,212018;江苏科技大学,生物与环境工程学院,江苏,镇江,212018;江苏科技大学,生物与环境工程学院,江苏,镇江,212018 |
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| 摘 要： | 采用酸碱法及加热法初步研究了提取樟树叶蛋白的工艺。结果表明:樟树叶蛋白的最佳提取工艺条件:常温下浸提剂的pH为5,料液比为1∶15,打浆时间4 min,浸提时间4 min。叶蛋白的分离条件:温度为90℃时,沉淀分离出叶绿体蛋白质,调节pH为3时分离出细胞质蛋白质Ⅰ,调节pH为11时分离出细胞质蛋白质Ⅱ。在此工艺条件下四季叶蛋白得率分别为6.22%、5.25%、15.05%和5.34%。
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| 关 键 词： | 樟树 叶蛋白 提取工艺 |
Dynamic Analysis on Extraction Technique and Four Seasons Content of Leaf Protein Concentrate from Camphor Trees |
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| WANG Na,CHU Yan-liang,LI Jing,WANG Kai-xuan.Dynamic Analysis on Extraction Technique and Four Seasons Content of Leaf Protein Concentrate from Camphor Trees[J].Northern Horticulture,2011(7):51-54. |
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| Authors: | WANG Na CHU Yan-liang LI Jing WANG Kai-xuan |
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| Institution: | WANG Na,CHU Yan-liang,LI Jing,WANG Kai-xuan(College of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212018) |
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| Abstract: | The optimum technique of extracting leaf protein concentrate(LPC) from the fresh leaves of camphor trees were studied with the methods of acid,alkali and heating.The results showed that under the normal temperature high yield of LPC could obtained by water(pH 5),solid-liquid ratio of 1∶15(g/mL),crushing leaves for 4 min and soaking for 4 min.The most optimum precipitating conditions were the temperature of 90℃ for the chloroplastic proteins,the pH 3 for cytoplasmic proteinsⅠ and the pH 11 for the cytoplasmic proteins Ⅱ.By this technique about 6.22,5.25,15.05,5.34 g LPC could been extracted from 100 g fresh leaves of the four seasons. |
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| Keywords: | camphor trees leaf protein concentrate(LPC) extraction technique |
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