| 禽呼肠孤病毒C-98分离株L1基因的克隆与序列分析 |
| |
| 引用本文： | 郝瑞峰,关平原,乌日罕,特木尔巴根,马立峰,于富丽,李瑞纲.禽呼肠孤病毒C-98分离株L1基因的克隆与序列分析[J].中国畜牧兽医,2008,35(9):37-40. |
| |
| 作者姓名： | 郝瑞峰 关平原 乌日罕 特木尔巴根 马立峰 于富丽 李瑞纲 |
| |
| 作者单位： | 1．内蒙古农业大学 动物科学与医学学院， 呼和浩特 010018；2．内蒙古动物疫病预防与控制中心， 呼和浩特 010010 |
| |
| 基金项目： | 内蒙古自然科学基金
|
| |
| 摘 要： | 参考禽呼肠孤病毒S1133株（GenBank）L1基因序列设计3对特异性引物，采用RT-PCR方法对禽呼肠孤病毒内蒙古分离株C-98的L1基因进行了克隆和序列测定。结果表明：获得的C-98 L1基因全长3959 bp，其中包括21 bp的5’非编码区和56 bp的3’非编码区，阅读框位于5’端第22位核苷酸与3’端第3903位核苷酸之间。将C-98株与国内外禽呼肠孤病毒群和哺乳动物呼肠孤病毒群的不同分离株进行序列比较，结果显示：C-98与台湾地区分离株919和美国分离株1733、2408、S1133亲缘关系最近，其核苷酸同源性分别为99.8%、99.7%、99.7%、99.4%；与哺乳动物呼肠孤病毒L3基因核苷酸及其编码的氨基酸同源性较低，为43%～45%。
|
| 关 键 词： | 禽呼肠孤病毒 L1基因 分子克隆 序列分析 |
Cloning and Sequence Analysis of the L1 Gene of Avian Reovirus C-98 Strain |
| |
| HAO Rui-feng,GUAN Ping-yuan,Wurihan,Temuerbagen,MA Li-feng,YU Fu-li,LI Rui-gang.Cloning and Sequence Analysis of the L1 Gene of Avian Reovirus C-98 Strain[J].China Animal Husbandry & Veterinary Medicine,2008,35(9):37-40. |
| |
| Authors: | HAO Rui-feng GUAN Ping-yuan Wurihan Temuerbagen MA Li-feng YU Fu-li LI Rui-gang |
| |
| Institution: | 1.College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China; 2.Center of Prevent and Control of Animal Disease of Inner Mongolia, Huhhot 010010, China |
| |
| Abstract: | Three specific pairs of primers, which were designed according to avian reovirus strain S1133 (GenBank) L1 gene sequence, were used to clone the L1 gene of the avian reovirus C-98 isolated from Inner Mongolia through RT PCR. Then the acquired L1 gene was sequenced. The result showed that there was 3959 bp in C-98 L1 gene, including 21 bp of the 5’ noncoding region and 56 bp of 3’ noncoding region. The reading frame was between the 22nd bp and the 3903rd bp. The comparison of the sequences of different strains showed higher homology between the strain C-98 and strain 919 from Taiwan district, strain 1733, 2408, S1133 from USA, and the homology of nucleotide sequence was 99.8%, 99.7%, 99.7% and 99.4% respectively; while there was a low relationship in comparison with the mammalian reovirus L3 genes and corresponding amino acid sequences, and the homology of nucleotide sequence was between 43% and 45%. |
| |
| Keywords: | avian reovirus L1 gene molecular cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
