| 硫酸化酵母葡聚糖对鸡球虫病的防治效果 |
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| 引用本文： | 刘博,陈融,陈凯,彭鼎,王米.硫酸化酵母葡聚糖对鸡球虫病的防治效果[J].畜牧兽医学报,2021,52(4):1126-1133. |
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| 作者姓名： | 刘博 陈融 陈凯 彭鼎 王米 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所农业农村部兽用化学药物及制剂学 重点实验室, 上海 200241;2. 长江大学, 荆州 434023 |
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| 基金项目： | 国家重点研发计划（2018YFE9102600） |
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| 摘 要： | 旨在探讨硫酸化酵母葡聚糖（sGSC）对人工感染鸡柔嫩艾美耳球虫（Eimeria tenella）病的防治效果。采用笼饲法，将200只鸡随机分为A组（4 mg·kg-1）、B组（16 mg·kg-1）、C组（64 mg·kg-1）、D组（感染对照组）和E组（健康对照组）。感染球虫前3 d开始，给A、B、C组鸡每天按体重饮水喂上述各浓度的sGSC，给D、E组的鸡喂纯水（RO水），持续至试验结束。试验过程中观察各组鸡的临床症状、血便情况并记录，收集感染后5～6 d的粪便，检测每克粪便中的卵囊数，感染后第7天剖杀所有鸡，计算脏器指数；并通过Illumina测序平台研究鸡盲肠内微生物群落多样性。结果显示：1）与D组比较，各给糖组鸡的盲肠肿胀明显减轻，肠内容物和出血明显减少，与E组鸡体重相比，A、D组体重明显降低（P<0.05），但B组和C组鸡的体重升高至接近E组水平，体重与E组鸡无显著差异（P>0.05）；B组和C组鸡排出的卵囊数与D组相比，出现了明显的减少，但无统计学差异（P>0.05）；A组鸡的胸腺指数明显增加（P<0.05），肝指数也出现显著改善（P<0.05）；2）对盲肠内菌群分析发现，sGSC能提高盲肠内韦荣氏菌科（Veillonellaceae）和乳酸杆菌科（Lactobacillaceae）的相对丰度，降低肠球菌科（Enterococcaceae）的相对丰度。sGSC能减轻柔嫩艾美尔球虫感染对鸡造成的危害，且随着sGSC剂量的增加，效果越明显。综上表明，sGSC具有一定的抗球虫效果，并且对感染球虫鸡肠道内的菌群具有有益的调节作用。
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| 关 键 词： | 硫酸化酵母葡聚糖 球虫 肠道菌 药物滥用 |
| 收稿时间： | 2020-09-15 |
Control Effects of Sulfated Yeast Glucan on Chicken Coccidiosis |
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| LIU Bo,CHEN Rong,CHEN Kai,PENG Ding,WANG Mi.Control Effects of Sulfated Yeast Glucan on Chicken Coccidiosis[J].Acta Veterinaria et Zootechnica Sinica,2021,52(4):1126-1133. |
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| Authors: | LIU Bo CHEN Rong CHEN Kai PENG Ding WANG Mi |
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| Institution: | 1. Key Laboratory of Veterinary Chemicals and Pharmaceutics, Shanghai Veterinary Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;2. Changjiang University, Jingzhou 434023, China |
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| Abstract: | The purpose of our research was to explore the effects of sulfated yeast glucan (sGSC) on the prevention and treatment of artificial Eimeria tenella disease in chickens. In this study, cage feeding was used to randomly divide 200 chickens into group A (4 mg·kg-1), group B (16 mg·kg-1), group C (64 mg·kg-1), group D (infection control group) and group E (healthy control group). Three days before the coccidiosis infection, the chickens of groups A, B, and C were given the above-mentioned concentrations of sGSC by body weight every day, and the chickens of groups D and E were fed with reverse osmosis water (RO water) until the end of the experiment. During the experiment, the clinical symptoms, and bloody stools of each group of chickens were observed and recorded, the feces from 5 to 6 days after infection were collected and the number of oocysts per gram of feces was detected. All chickens were killed on the 7th day after infection, and the organ index was calculated; The diversity of the microbial community in the cecum of the chicken was studied through the Illumina sequencing platform. The results showed that： 1) Compared with group D, the cecal swelling of the chickens in each sugar group was significantly reduced, and their intestinal contents and bleeding were significantly reduced. Compared with the weight of the chickens in the E group, the weight of the A and D groups was significantly reduced (P<0.05), however, the body weight of chickens in group B and C were increased to the level of group E, and there was no significant difference with the body weight from chickens in group E (P>0.05); the number of oocysts excreted by chickens in group B and C was compared with that in group D, and there have a significant reduction in group B and C compared with group D, but there was no statistical difference (P>0.05); The thymus index of chickens in group A increased significantly (P<0.05), and the liver index was also significantly improved (P<0.05); 2) Analysis of the cecal flora found that sGSC increased the relative abundance of Veillonellaceae and Lactobacillaceae in the cecum. The relative abundance of Enterococcaceae has been reduced. The results showed that sGSC could reduce the damage caused by Eimeria tenella infection to chickens, and the effects were more obvious with the increase of sGSC dose. In short, sGSC has good anti-coccidial effects, and has beneficial regulatory effects on the intestinal flora of chickens infected with coccidia. In conclusion, sGSC has a certain anti-coccidial effect and has beneficial regulatory effect on the intestinal flora of chickens infected with coccidia. |
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| Keywords: | sulfated yeast glucan coccidia intestinal bacteria overuse of drugs |
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