| 2017—2019年华东地区猪场主要病毒性腹泻病原调查 |
| |
| 引用本文： | 常新见,周金柱,殷杰,牛贝贝,范宝超,郭容利,赵永祥,牛家强,何孔旺,李彬.2017—2019年华东地区猪场主要病毒性腹泻病原调查[J].畜牧兽医学报,2020,51(12):3141-3150. |
| |
| 作者姓名： | 常新见 周金柱 殷杰 牛贝贝 范宝超 郭容利 赵永祥 牛家强 何孔旺 李彬 |
| |
| 作者单位： | 1. 江苏省农业科学院兽医研究所, 南京 210014;2. 西藏农牧学院动物科学学院, 林芝 860000;3. 内蒙古民族大学动物科学技术学院, 通辽 028000;4. 南京农业大学动物医学院, 南京 210014 |
| |
| 基金项目： | 国家重点研发计划（2018YFD0500101）；国家自然科学基金（31872481）；江苏省人兽共患病学重点实验室资助项目（R1907） |
| |
| 摘 要： | 本研究旨在了解当前我国华东地区引起猪腹泻的主要病毒性腹泻病原的流行情况，为该地区更好地开展猪腹泻病毒的防控工作提供临床数据。分别采用RT-PCR检测方法对2017—2019年华东地区35个场别594份临床样品检测猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）、猪轮状病毒（porcine rotavirus，PoRV）、猪传染性胃肠炎病毒（transmissible gastroenteritis virus of swine，TGEV）、猪丁型冠状病毒（porcine deltacoronavirus，PDCoV）和猪萨佩罗病毒（porcine Sapelovirus，PSV）等5种猪的病毒性腹泻病原，并对其阳性率及混合感染情况进行统计分析。结果显示，2017—2019年的PEDV、PDCoV、PoRV、TGEV及PSV的平均阳性率为分别为62.6%（372/594）、27.9%（166/594）、16.8%（100/594）、13.3%（79/594）及3.87%（23/594），二重混合感染中以PEDV+PDCoV及PEDV+PoRV为主，平均混合感染率分别为22.4%（133/594）和13.3%（79/594）。三重感染中以PEDV+PoRV+PDCoV混合感染为主，感染率为7.58%（45/594），未见四重及五重病原混合感染。2017—2019年PEDV平均阳性检出率最高，且常与PoRV及PDCoV发生混合感染，是当前华东地区猪病毒性腹泻类疫病的防控重点。TGEV和PDCoV在健康育肥猪及母猪的检出率中占比较大，表明隐性感染变得普遍，值得养殖户关注。除此之外，PSV的检出率远低于其他4种腹泻病毒。总之，随着2018年后养殖场加强生物安全防控措施和猪群管理，各病原检出率和阳性场检出率呈现逐年下降趋势。
|
| 关 键 词： | 猪病毒性腹泻 PEDV 混合感染 华东地区 |
| 收稿时间： | 2020-03-16 |
Investigation on Pathogens of Major Viral Diarrhea in Pig Farms in East China from 2017 to 2019 |
| |
| CHANG Xinjian,ZHOU Jinzhu,YIN Jie,NIU Beibei,FAN Baochao,GUO Rongli,ZHAO Yongxiang,NIU Jiaqiang,HE Kongwang,LI Bin.Investigation on Pathogens of Major Viral Diarrhea in Pig Farms in East China from 2017 to 2019[J].Acta Veterinaria et Zootechnica Sinica,2020,51(12):3141-3150. |
| |
| Authors: | CHANG Xinjian ZHOU Jinzhu YIN Jie NIU Beibei FAN Baochao GUO Rongli ZHAO Yongxiang NIU Jiaqiang HE Kongwang LI Bin |
| |
| Institution: | 1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China;3. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;4. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China |
| |
| Abstract: | To investigate the epidemic situation of viral diarrhea in East China in recent years, and provide epidemiological survey data for comprehensive prevention, 549 fecal samples were collected in 2017-2019 from diarrhea pigs in 5 cities of East China and RT-PCR were used to detect porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PoRV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV) and porcine Sapelovirus (PSV), and statistical analysis of its positive rate and mixed infection was performed.The results showed that during 2017 and 2019, PEDV,PDCoV,PoRV,TGEV and PSV positive rate were 62.6% (372/594),27.9% (166/594),16.8% (100/594),13.3% (79/594), and 3.87% (23/594), respectively.In the double mixed infection, the average mixed infection rate of PEDV + PDCoV and PEDV + PoRV were the main ones, which were 22.4% (133/594) and 13.3% (79/594), respectively. Among the triple infections, the mixed infection rate of PEDV + PoRV + PDCoV was dominated by 7.58% (45/594), and there were no mixed infections of four and five pathogens. The highest positive detection rate of PEDV was during 2017 and 2019, and mixed infection with PoRV and PDCoV, which was currently the focus of prevention and control of viral diarrhea in East China. TGEV and PDCoV accounted for a large proportion of the detection rate of healthy fattening pigs and sows, indicating that recessive infections had become common and deserve attention from farmers. In addition, the detection rate of PSV was more lower than the other four diarrhea viruses. On the whole, since August 2018 that the farms strengthened biosecurity prevention and control measures, the detection rate of various pathogens and the detection rate of sites have shown a downward trend year by year. |
| |
| Keywords: | porcine viral diarrhea PEDV mixed infection East China |
|
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
| 点击此处可从《畜牧兽医学报》下载免费的PDF全文 |
|
