| ~(60)Coγ射线对小豆种子辐射处理效应的研究 |
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| 引用本文： | 金文林,陈学珍,喻少帆.~(60)Coγ射线对小豆种子辐射处理效应的研究[J].核农学报,2000,14(3):134-140. |
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| 作者姓名： | 金文林 陈学珍 喻少帆 |
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| 作者单位： | 北京农学院作物遗传育种研究所,北京,102206 |
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| 基金项目： | 北京市科干局青年科技骨干培养基金 |
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| 摘 要： | 本试验研究结果表明 :60 Coγ射线辐照小豆种子的半致死剂量平均为 3 89Gy ,且M1生长初期叶片性状有明显的叶面缺刻、叶色缺绿、叶形不对称、叶缘卷曲、花叶、黄叶等致变现象 ,各种畸型叶出现频率与辐照剂量大小有关 ;M1根尖细胞中有多种染色体畸变类型 ,以染色体桥和断片频率较高 ,辐照剂量在 40 0Gy以上时根尖细胞异常率达 1 0 %以上 ;植株花粉育性与辐照剂量呈极显著负相关 ,京农 2号植株花粉育性达 5 0 %时的辐照剂量为 3 78Gy。根据M1出苗率、成活株率、根尖细胞异常率、叶片致畸率、植株花粉育性、植株农艺性状受抑程度及M2 植株性状的变异等综合分析 ,60 Coγ射线辐照小豆种子的较适宜剂量为 40 0Gy
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| 关 键 词： | 小豆 ~(60)Coγ射线 适宜辐照剂量 遗传变异 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
A STUDY ON THE RADIATIVE MUTATION ON THE SEEDS OF ADZUKI BEAN BY ~(60)Co γ RAY |
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| JIN Wen-lin,CHEN Xue-zhen,YU Shao-fan.A STUDY ON THE RADIATIVE MUTATION ON THE SEEDS OF ADZUKI BEAN BY ~(60)Co γ RAY[J].Acta Agriculturae Nucleatae Sinica,2000,14(3):134-140. |
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| Authors: | JIN Wen-lin CHEN Xue-zhen YU Shao-fan |
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| Abstract: | The seeds of Adzuki bean were irradiated by 60 Co γ ray of 0～700Gy.The results showed that:(1)The sub\|lethal dose(LD 50 )was 389Gy and the early seedling leaf at M\-1 showed incised,chlorotic,yellow,mosaicand curly edge.The absorbed dose was related with the rate of misshaped leaf.Chromosomal aberration, chromosome bridge and fragment of M\-1 in the tip were observed.If the seed was irradiated with 400Gy,the rate of abnormal cell at the root was more than 10%.The relationship between pollen fertility and irradiation dose was negative correlated.When the irradiation dose was 387 Gy,the pollen fertility was 50% in the seeds of Adzuki bean.(2)The optimum irradiation dose for the seed of Adzuki bean is 400Gy in the M\-1 and M\-2. |
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| Keywords: | Adzuki bean 60Coγray optimum dose variation |
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