| 菊属种间杂种的鉴定 |
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| 引用本文： | 李辛雷,陈发棣,崔娜欣.菊属种间杂种的鉴定[J].南京农业大学学报,2005,28(1):24-28. |
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| 作者姓名： | 李辛雷 陈发棣 崔娜欣 |
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| 作者单位： | 南京农业大学园艺学院,江苏,南京,210095 |
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| 基金项目： | 江苏省基础计划青年创新人才基金项目 (BK2002417)，江苏省教育厅高技术产业化项目 (JH02 086 )，江苏省三项工程项目 [S01 (48) 和SX (2003) 065]，江苏省高技术研究项目 (BG2003305) |
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| 摘 要： | 利用RAPD技术、细胞学及形态学手段对菊属种间杂种进行鉴定。RAPD标记结果表明：杂种表现出父本特异带或双亲均不具备的新谱带。细胞学鉴定结果表明：杂种染色体数目为亲本平均值。形态学性状观察、统计表明：大多数杂种性状介于双亲之间，但部分性状则较为明显地相似于多倍体；部分杂种表现父本特异性状，或出现双亲所不具备的新性状；二倍体野生种与栽培菊花杂种形态分离广泛，说明多倍体栽培菊花具复杂的遗传背景。
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| 关 键 词： | 菊属 种间杂种 RAPD 细胞学 形态学 鉴定 |
| 文章编号： | 1000-2030(2005)01-0024-05 |
| 修稿时间： | 2004年4月6日 |
Identification of interspecific hybrids in Dendranthema |
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| LI Xin-lei,CHEN Fa-di,CUI Na-xin.Identification of interspecific hybrids in Dendranthema[J].Journal of Nanjing Agricultural University,2005,28(1):24-28. |
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| Authors: | LI Xin-lei CHEN Fa-di CUI Na-xin |
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| Institution: | LI Xin-lei,CHEN Fa-di *,CUI Na-xin |
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| Abstract: | RAPD,cytological and morphological analysis were used for the identification of interspecific hybrids in Dendranthema.RAPD results indicated that the hybrids produced specific bands of male parent or new bands absent in both parents.The results of cytological analysis indicated that chromosome number of F 1 hybrids was the average of their parents.The observation and statistics of morphology suggested that the hybrids showed interspecific morphological traits and some traits resembled more closely with the higher polyploidy parents.Some hybrids showed specific morphological traits of their male parent or unique morphological traits their parents did not possess.The morphological traits of hybrids between diploids and chrysanthemum cultivars varied in a large range,indicating that the polyploid cultivated chrysanthemum contained complexed genetic background. |
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| Keywords: | Dendranthema interspecific hybrid RAPD cytology morphology identification |
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