| 运用致病毒素筛选抗烟草黑胫病细胞突变体I抗毒素愈伤组织的有效筛选 |
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| 引用本文： | 王荔,罗文富,杨艳琼,杨德.运用致病毒素筛选抗烟草黑胫病细胞突变体I抗毒素愈伤组织的有效筛选[J].云南农业大学学报,1999(1). |
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| 作者姓名： | 王荔 罗文富 杨艳琼 杨德 |
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| 作者单位： | 云南农业大学农业科学技术学院 |
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| 摘 要： | 以６０Ｃｏ－γ射线照射烟草优质感病品种的花药作为诱变手段，用烟草黑胫病疫霉菌粗毒素为筛选剂，对烟草花粉植株叶片的愈伤组织，进行抗病突变体细胞的筛选．试验表明：毒素对烟草愈伤组织的存活和增殖有明显的抑制作用，抑制程度随毒素浓度提高而加强，同一浓度对不同品种也存在着差异．综合毒素浓度对烟草愈伤组织抑制、增殖程度及筛选的有效性，初步认为：筛选红大和Ｋ３２６抗黑胫病突变体细胞的最适粗毒素浓度分别为３０％和４５％．所筛选出的抗病突变体细胞，其细胞水平的抗性与再生植株的抗病性鉴定相吻合．
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| 关 键 词： | 烟草 黑胫病 毒素 愈伤组织 突变体筛选 诱变 |
Using Phytotoxin to select Mutants Resistant to Tobacco Phytophthora parasitia var.nicotinae
Selection of Callus Resistant to Phytotoxin |
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| Wang LiLuo WenfuYang yanqiongYang De.Using Phytotoxin to select Mutants Resistant to Tobacco Phytophthora parasitia var.nicotinae
Selection of Callus Resistant to Phytotoxin[J].Journal of Yunnan Agricultural University,1999(1). |
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| Authors: | Wang LiLuo WenfuYang yanqiongYang De |
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| Abstract: | THe anthers form tobacco varieties with high quality and high sensitivity to tobacco
disease Phytophthora parasitica var nicotina were induced to mutate by -ray of60Co and then
were regenerated into pollen plants through tissue culture.The callus originated from the leaves
of pollen plants were cultured in medium complemented with phytotoxin extracted from
Phytophthora parasitica var nicotina to select cell mutants resistant to this disease.The results
indicate that:the extracted phytotoxin has a significant inhibition on the activity and propagation
of tobacco callus;the effect will increase with rising concentration of phytotoxin added in
medium;different varieties show various responses to the same concentration.Based on the
results,it has been believed that the optimal concentration of phytotoxin is respectively 30%
and 40% for selecting cell mutants resistant to the disease in Hongda and K326 varieties.There
is an agreement in disease resistance between cell mutants and whole plant mutants rege |
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| Keywords: | Tobacco Phytophthora parasitica var nicotina Phytotoxin Callus selection of mutant Inductive variation nerated |
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