| 纤维素酶活力测定条件研究 |
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| 引用本文： | 夏服宝,邱雁临,孙宪迅.纤维素酶活力测定条件研究[J].饲料工业,2005,26(16):23-26. |
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| 作者姓名： | 夏服宝 邱雁临 孙宪迅 |
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| 作者单位： | 湖北工业大学生物工程学院 |
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| 摘 要： | 用DNS法测定纤维素酶活力,分析测定条件对测定结果的影响。在不同的条件下测定酶活力会得到不同的纤维素酶活力测定值。其中,酶液用量、酶解反应时间、温度对测定结果影响较大,酶液的稀释度、DNS显色时间对测定结果也有一定影响。测定纤维素酶活力的最适条件为:酶解反应时间为30min,最适温度40℃,DNS用量2.5ml,显色时间5min。
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| 关 键 词： | 纤维素酶 酶活力 测定条件 |
| 收稿时间： | 2005-02-25 |
| 修稿时间： | 2005年2月25日 |
Studies on Condition for Determination of Cellulase Activity |
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| Xia Fubao,Qiu Yanlin,Sun Xianxun.Studies on Condition for Determination of Cellulase Activity[J].Feed Industry,2005,26(16):23-26. |
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| Authors: | Xia Fubao Qiu Yanlin Sun Xianxun |
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| Abstract: | The purpose of this study is analyzing the effects of determination con ditions on results of measurements of by the DNS method. On different conditions , the results of the measurements of cellulase activity are different. Among the se conditions, the concentration of cellulase, reactions time and temperature ha ve great effects on the results. The optimum conditions for determination of cel lulase activity: 30min for enzymatic reaction;optimum temperature 40℃,the quant ity of DNS is 2.5ml and 5min for cloring. |
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| Keywords: | cellulase cellulase activity determination |
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