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杏果实发育成熟过程中细胞壁组分和水解酶活性的变化
引用本文:李萍,廖康,赵世荣,安晓芹,刘娟,李楠,成小龙.杏果实发育成熟过程中细胞壁组分和水解酶活性的变化[J].新疆农业大学学报,2013(4):298-303.
作者姓名:李萍  廖康  赵世荣  安晓芹  刘娟  李楠  成小龙
作者单位:新疆农业大学特色果树研究中心,乌鲁木齐830052
基金项目:新疆维吾尔自治区重大专项(201130102);新疆维吾尔自治区科技支撑项目(200931101);新疆维吾尔自治区果树重点学科
摘    要:以早、中、晚熟6个新疆杏品种为材料,测定果实坐果至成熟过程中细胞壁含量、细胞壁组成物质含量、果胶酶、纤维素酶和口一葡萄糖苷酶活性的变化,深入探究杏果实发育成熟生理机制。研究结果表明:杏果实发育成熟过程中,细胞壁含量下降;水溶性和离子型果胶含量上升,早熟品种巴都玉吕克水溶性果胶含量最高,成熟时为13.26mg/g,中熟品种轮台小白杏最低,为10.60mg/g,离子型果胶含量与其相似,两品种间差异显著(P〈0.05);共价结合果胶含量呈先上升,至成熟期下降的趋势;纤维素和半纤维素含量整体呈下降趋势,至成熟期,纤维素下降至原含量的1/3左右;不同品种杏果胶酶活性大小依次为:早熟品种〉中熟品种〉中晚熟品种,品种间差异显著(P〈0.05);纤维素酶活性整体由0.47U/g左右上升至1.42U/g左右,品种间差异不显著;p葡萄糖苷酶活性变化规律性不强,品种间差异不显著(P〉0.05);其中,早、中及中晚熟果实果胶酶活性依次递减,且差异显著(P〈0.05)。

关 键 词:  果实发育  细胞壁  果胶  纤维素  水解酶

Variation of Cell Wall and Hydrolases in Apricot Fruit During Development and Maturing Process
LI Ping,LIAO Kang,ZHAO Shi-rong,AN Xiao-qin,LIU Juan,LI Nan,CHENG Xiao-long.Variation of Cell Wall and Hydrolases in Apricot Fruit During Development and Maturing Process[J].Journal of Xinjiang Agricultural University,2013(4):298-303.
Authors:LI Ping  LIAO Kang  ZHAO Shi-rong  AN Xiao-qin  LIU Juan  LI Nan  CHENG Xiao-long
Institution:(Research Center of Featured Fruit Tree, Xinjiang Agricultural University, Urumqi 830052,Chi- na)
Abstract:The early varieties, medium varieties and later medium varieties in Xinjiang apricot cultivars were used as materials to analyze the changes of cell wall content,cell wall components content, PG,cellu- lase and β-glycosidase during fruit development and maturation process delve into the maturation physio- logical mechanisms of apricot fruit.The result showed: during fruit development and ripening period, cell wall content decreased, soluble pectin and ionic pectin content increased, in ripening period, baduyulvke contained the highest soluble pectin, was 13. 26 rag/g, Luntaixiaobaixing contained the lowest, was 10.60 mg/g,ionic pectin content was similar to the soluble pectin, there were significant differences be- tween the two varieties (P 〈0.05), covalently bound pectin content increased first,in maturity showed downward trend,cellulose and hemicellulose showed downward trend in whole,in ripening period,cellulose content decreased to about 1/3 of the original content,different varieties of apricots' pectinase activity se- quence were as follows: early maturing varieties〉 medium maturing variety〉 late maturing varieties, there were significant differences between the varieties (P 〈0.05),cellulase activity entirety rosed from 0.47 U/g around by 1.42 U/g around there were , no significant differences among varieties , variation regularity of β-glycosidase activity was not clear,there were no significant differences among varieties.
Keywords:apricot  fruit development  cell wall  pectin  cellulose  hydrolase
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