| 低温淀粉酶高产菌株的诱变选育 |
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| 引用本文： | 古丽娜孜,库米拉,张晓燕,高雯君,李晓,王玮玥,武运.低温淀粉酶高产菌株的诱变选育[J].新疆农业大学学报,2013(4):288-292. |
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| 作者姓名： | 古丽娜孜 库米拉 张晓燕 高雯君 李晓 王玮玥 武运 |
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| 作者单位： | 新疆农业大学食品科学与药学学院,乌鲁木齐830052 |
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| 基金项目： | 新疆维吾尔自治区科技支疆项目（20101230750） |
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| 摘 要： | 以一株解淀粉芽孢杆菌（Bacillus a myloliquefaciens）A-4为出发菌株，采用紫外线（UV）和亚硝基胍（NTG）诱变，以期筛选出高产低温淀粉酶量诱变菌株；经紫外线诱变、亚硝基胍诱变、紫外线一亚硝基胍复合诱变，所得突变菌株经淀粉酶平板水解圈初筛、再经摇瓶发酵复筛，得高产低温淀粉酶突变株UNA46，其最大产酶量62．13U／mL，是出发菌株A-4的1．96倍；将UNA4-6连续传代6次，低温淀粉酶活性仍可达到第一代的99．7％，试验表明遗传稳定性好。
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| 关 键 词： | 低温淀粉酶 紫外诱变 亚硝基胍诱变 复合诱变 |
Mutagenesis Breeding of High-Yielding Strains from Low-Temperature Amylase |
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| Gulinazi,Kumila,ZHANG Xiao-yan,GAO Wen-jun,LI Xiao,WANG Wei-yue,WU Yun.Mutagenesis Breeding of High-Yielding Strains from Low-Temperature Amylase[J].Journal of Xinjiang Agricultural University,2013(4):288-292. |
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| Authors: | Gulinazi Kumila ZHANG Xiao-yan GAO Wen-jun LI Xiao WANG Wei-yue WU Yun |
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| Institution: | (College of Food Science and Pharmaceutical Science, Xinjiang Agricultural University, Urumq 830052 ,China) |
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| Abstract: | As an original strain, an amyloliquefaciens Bacillus （Bacillus arnyloliquefaciens） A4 preserved in our laboratory was mutated by ultraviolet ray and nitroso guanidine respectively, the optimal induced mutation conditions were gained.A mutant strain named UNA4-6 with high-yielding low-temperature am- ylase activity was obtained though the screening of amylase flat and the rescreening of flask fermentation. The maximum amylase production of UNA4-6 was 1.96 times more than that of the original strain,The greatest amylase yield was 62.13 U/mL.If UNA4-6 were continuously handed from generation to genera- tion for 6 times,the activity of low-temperature amylase still can reach 99.7% of the first generation.The subcultures tests showed UNA4-6 has good genetic stability. |
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| Keywords: | low temperature amylase ultraviolet mutagenesis nitrosoguanidine mutagenesis |
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