| 苜蓿褐斑病接种方法研究 |
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| 引用本文： | 宋卫玲,袁庆华.苜蓿褐斑病接种方法研究[J].中国草地学报,2006,28(5):38-41. |
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| 作者姓名： | 宋卫玲 袁庆华 |
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| 作者单位： | 中国农业科学院畜牧研究所,北京,100094 |
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| 摘 要： | 以苜蓿假盘菌为接种菌源,对3个不同抗性的苜蓿品种(即伊鲁瑰斯、沙湾和泾阳)采用3种活体整株接种方法,即接种盘倒扣接种法、活体病株覆盖接种法和孢子悬浮液喷雾接种法,进行比较,以筛选出稳定、可靠、操作简便、发病速度较快的接种方法.结果表明,病株覆盖接种和孢子悬浮液喷雾接种为较理想的接种方法,可以比较准确、快速的鉴定出品种及单株的抗病性,其中孢子悬浮液最佳缓冲液组成为:0.5%吐温-20 4.5%葡萄糖 9mg/L萘乙酸 1g/L菌落.
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| 关 键 词： | 苜蓿 褐斑病 接种方法 |
| 文章编号： | 1673-5021(2006)05-0038-04 |
| 修稿时间： | 2006年1月24日 |
Study on Inoculation Methods of Common Leaf Spot in Alfalfa |
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| SONG Wei-ling,YUAN Qing-hua.Study on Inoculation Methods of Common Leaf Spot in Alfalfa[J].Chinese Journal of Grassland,2006,28(5):38-41. |
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| Authors: | SONG Wei-ling YUAN Qing-hua |
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| Abstract: | Three methods of whole alive plant inoculation(inverse plate of inoculation,sowing fungus plants,spore spraying) were studied to identify alfalfa resistance to common leaf spot.Three varieties of alfalfa(Medicago sativa L.cv.Iroquois,M.sativa L.cv.Shawan,M.sativa L.cv.Jingyang) with known difference in resistance to P.medicaginis were inoculated with Pseudopeziza medicaginis.The results showed that spore suspension spraying is the most stability and more quick one of the whole plant inoculation methods.The methods can quickly,well and truly screened the varieties of alfalfa resistance.The best spore suspension is composed of 0.5%Tween-20 +4.5%D-Glucose+9mg/L 1-aphthylacetic acid +1g/L spore. |
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| Keywords: | Alfalfa Common leaf spot Inoculation methods |
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