| 适宜大叶黄杨茎段不同组织培养阶段的培养基研究 |
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| 引用本文： | 林海.适宜大叶黄杨茎段不同组织培养阶段的培养基研究[J].西部林业科学,2006,35(2):102-104. |
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| 作者姓名： | 林海 |
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| 作者单位： | 鹤壁职业技术学院,河南,鹤壁,458030 |
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| 摘 要： | 以MS为基本培养基,附加不同种类、浓度的生长调节剂,通过试验寻求适宜于大叶黄杨茎段各组织培养阶段的培养基。研究结果表明:在诱导培养阶段,较低浓度的细胞分裂素能促进大叶黄杨茎段侧芽的萌发,其适宜的诱导培养基为MS BA0.5 mg/L;继代增殖阶段,以MS BA1.0 mg/L NAA0.1 mg/L GA32.0 mg/L为培养基效果较好;生根阶段,最适宜的培养基为1/2 MS NAA0.5 mg/L。
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| 关 键 词： | 大叶黄杨 茎段外植体 组织培养 培养基 |
| 文章编号： | 1672-8246(2006)02-0102-03 |
| 收稿时间： | 2005-11-10 |
| 修稿时间： | 2005年11月10 |
A Study on Stem Tissue Culture Medium Suitable for Different Culture Stage of Buxus megistophylla |
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| LIN Hai.A Study on Stem Tissue Culture Medium Suitable for Different Culture Stage of Buxus megistophylla[J].Journal of West China Forestry Science,2006,35(2):102-104. |
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| Authors: | LIN Hai |
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| Institution: | Hebi College of Vocation and Technology, Hebi Hunan 458030, P. R. China |
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| Abstract: | Taking MS as the basic medium,adding different plant growth regulators of different concentrations,the tissue culture medium of Buxus megistophylla at different culture stage was studied.The resultsof study indicated that at the inducing stage,low concentrated cytokinin was benefit for the formation of lateral bud,and the suitable medium was MS+BA 0.5 mg/L,the suitable medium for subculture was MS+BA 1.0 mg/L+NAA 0.1 mg/L+GA_(3)2.0 mg/L,and the recommended medium for rooting stage was 1/2 MS+ NAA 0.5 mg/L. |
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| Keywords: | Buxus megistophylla stem plantlet tissue culture medium |
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