Sequential combinations of C-banding and in situ hybridization and their use in the detection of interspecific introgressions into wheat |
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| Authors: | Angeles Cuadrado Pilar Rubio Esther Ferrer Nicolás Jouve |
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| Institution: | (1) Department of Cellular Biology and Genetics, University of Alcalá de Henares, E-28871 Alcalá de Henares, Madrid, Spain |
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| Abstract: | Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites. |
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| Keywords: | C-banding FISH heterochromatin in situ hybridization rye Secale cereale Secale montanum Triticum aestivum wheat |
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