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观音莲的组织培养
引用本文:魏贤彪,欧阳少林,胡庆.观音莲的组织培养[J].福建林业科技,2005,32(4):108-110.
作者姓名:魏贤彪  欧阳少林  胡庆
作者单位:1. 江西省吉安市森林资源监测中心,江西,吉安,343000
2. 江西省吉安市林业科学研究所,江西,吉安,343011
摘    要:观音莲的顶芽或侧芽在MS+6-BA2.0 mg.L-1+TDZ0.03 mg.L-1培养基中,可诱导产生大量丛生泡叶状不定芽。以此作为增殖培养,每28 d增殖率达6.0以上。壮芽培养基宜用MS+6-BA0.3 mg.L-1+Y(多效唑)0.2 mg.L-1,周期30~35d,由此获得的合乎生根标准的不定芽比率约80%左右。切取3 cm以上具完全展开叶的芽在MS+6-BA0.3 mg.L-1+Y(多效唑)0.2 mg.L-1培养基中,根的诱导率(生根率)达100%。

关 键 词:组织培养  观音莲
文章编号:1002-7351(2005)04-0108-03
收稿时间:2005-09-12
修稿时间:2005-09-122005-10-31

Alocasia amazonica Tissue Culture
WEI Xian-biao,OUYANG Shao-lin,HU Qing.Alocasia amazonica Tissue Culture[J].Journal of Fujian Forestry Science and Technology,2005,32(4):108-110.
Authors:WEI Xian-biao  OUYANG Shao-lin  HU Qing
Abstract:Alocasia amazonica top and lateral buds might be induced to produce large amount of fasciculate cystenchyma adventitious buds in the culture medium MS+6-BA2.0 mg·L~(-1)+TDZ0.03 mg·L~(-1).The proliferation rate reached 6.0 and more every 28 days by taking it for the proliferation culture.The vigorous bud culture medium is suitable by MS+6-BA0.3 mg·L~(-1)+Y0.2 mg·L~(-1),the cycle is 30~35 days long,the rate of adventitious bud obtained by the above way and suitable for the rooting standard was about 80%.The root inducing rate was 100% by cutting 3cm and longer of bud with wholly outspread leaf to put it into the culture medium MS+6-BA0.3 mg·L~(-1)+Y0.2 mg·L~(-1),the root inducing rate reached 100%.
Keywords:tissue culture  Alocasia amazonica
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