| 分子标记法和形态学特征对杏仁栽培种及野生种基因型的遗传关系构建的辨别 |
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| 引用本文： | Karim Sorkheh,Behrouz Shiran,Soghra Kiani,Nazanin Amirbakhtiar,Sadegh Mousavi,Vahid Rouhi,Shahram Mohammady-D,Thomas M.Gradziel,Lyudmyla V.Malysheva-Otto,Pedro Martínez-Gómez.分子标记法和形态学特征对杏仁栽培种及野生种基因型的遗传关系构建的辨别[J].林业研究,2009,20(3):183-194. |
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| 作者姓名： | Karim Sorkheh Behrouz Shiran Soghra Kiani Nazanin Amirbakhtiar Sadegh Mousavi Vahid Rouhi Shahram Mohammady-D Thomas M.Gradziel Lyudmyla V.Malysheva-Otto Pedro Martínez-Gómez |
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| 作者单位： | Karim Sorkheh,Behrouz Shiran,Nazanin Amirbakhtiar,Sadegh Mousavi,Vahid Rouhi,Shahram Mohammady-D(Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, Shahrekord 8818634141, P.O.Box 115, Iran);Soghra Kiani(Scientific assistance of Payam-e-Nour University, Farokhshahr8831871337, Iran);Thomas M. Gradziel(Department of Plant Science, University of California, Davis, CA 93616, USA);Lyudmyla V. Malysheva-Otto(Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr.3, Gatersleben 06466, Germany);Pedro Martínez-Gómez(Department of Plant Breeding, CEBAS-CSIC, P.O. Box 164, Espinardo 30080, Murcia, Spain) |
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| 摘 要： | 应用23个形态学特征,19个扩增片段长度多态性(AFLP)引物组合,80个随机扩增多态DNA(RAPD)引物和32个简单序列重复(SSR)引物对,比较三种分子标记法在29个杏仁栽培种和3个野生种遗传关系构建中的信息景和效率.根据预期杂合度的评价,与AFLPs和RAPDs相比,SSRs具有较高水平的多态性和较大的信息量.AFLPs预期朵合度值最低,但其辨别效率值最高,因为AFLPs能揭示每个反应中的大量条带,导致各种类型的多样性指数均较高.三种分子标记法对杏仁基因型的辨别效率均较高,只是SSRs无法辨别‘Monagha'和‘Sefied'杏仁基因型.三种分子标记法基因型相似性相关系数统计上显著,但SSR数据要低于RAPDs和AFLPs的值.尽管三种分子标记法树形图拓扑结构存在一些差异,但相似性水平均较高.SSRs、RAPDs和AFLPs的系统树图及其综合数据都能依据地理散布反映大多数栽培种的关系.AMOVA检测到每个地理组中栽培种和野生种的变异.辅助程序分析表明,实验所应用的标记物数量足以保证基因相似性估计的可靠性和标记法间的比较是有意义的.
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| 关 键 词： | 扩增片段长度多态性 随机扩增多态DNA 简单序列重复 种质 遗传关系 杏仁 |
| 收稿时间： | 2 November 2006 |
Discriminating ability of molecular markers and morphological characterization in the establishment of genetic relationships in cultivated genotypes of almond and related wild species |
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| Karim Sorkheh,Behrouz Shiran,Soghra Kiani,Nazanin Amirbakhtiar,Sadegh Mousavi,Vahid Rouhi,Shahram Mohammady-D,Thomas M. Gradziel,Lyudmyla V. Malysheva-Otto,Pedro Martínez-Gómez.Discriminating ability of molecular markers and morphological characterization in the establishment of genetic relationships in cultivated genotypes of almond and related wild species[J].Journal of Forestry Research,2009,20(3):183-194. |
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| Authors: | Karim Sorkheh Behrouz Shiran Soghra Kiani Nazanin Amirbakhtiar Sadegh Mousavi Vahid Rouhi Shahram Mohammady-D Thomas M Gradziel Lyudmyla V Malysheva-Otto Pedro Martínez-Gómez |
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| Institution: | (1) Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, Shahrekord, 8818634141, P.O.Box 115, Iran;(2) Scientific assistance of Payam-e-Nour University, Farokhshahr, 8831871337, Iran;(3) Department of Plant Science, University of California, Davis, CA 95616, USA;(4) Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr.3, Gatersleben, 06466, Germany;(5) Department of Plant Breeding, CEBAS-CSIC, P.O. Box 164, Espinardo, Murcia, 30080, Spain |
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| Abstract: | A total 23 morphological traits, 19 AFLP-primer combinations, 80 RAPD primers and 32 SSR primer pair were used to compare
the informativeness and efficiency of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP)
and simple sequence repeat (SSR) markers in establishing genetic relationships among 29 almond cultivars and three related
wild species. SSRs presented a high level of polymorphism and greater information content, as assessed by the expected hetrozygosity,
compared to AFLPs and RAPDs. The lowest values of expected hetrozygosity were obtained for AFLPs; however AFLPs showed the
highest efficiency, owing to their capacity to reveal large numbers of bands per reaction, which led to high values for various
types of indices of diversity. All the three techniques discriminated almond genotypes very effectively, except that SSRs
failed to discriminate between ‘Monagha’ and ‘Sefied’ almond genotypes. The correlation coefficients of similarity were statistically
significant for all the three marker systems, but were lower for the SSR data than for RAPDs and AFLPs. For all the markers,
high similarity in dendrogram topologies was obtained, although some differences were observed. All the dendrograms, including
that obtained by the combined use of all the marker data, reflect relationships for most of cultivars according to their geographic
diffusion. AMOVA detected more variation among cultivated and related wild species of almond within each geographic group.
Bootstrap analysis revealed that the number of markers used was sufficient for reliable estimation of genetic similarity and
for meaningful comparisons of marker types. |
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| Keywords: | Amplified Fragment Length Polymorphisms (AFLPs) Random Amplified Polymorphic DNA (RAPDs) Simple-Sequence Repeats (SSRs) germplasm genetic relationships breeding prunus dulcis |
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