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| 云南水稻品种原生质体培养研究 | |
| 引用本文： | 张尧忠,徐宁生,曾黎琼,松岗诚,伊势一男.云南水稻品种原生质体培养研究[J].西南农业学报,2001,14(1):16-19,30. |
| 作者姓名： | 张尧忠 徐宁生 曾黎琼 松岗诚 伊势一男 |
| 作者单位： | 1. 云南省农业科学院, 2. 日本中国农业实验场 3. 日本国际农业合作研究中心 |
| 摘 要： | 采用KPR和R2培养基进行水稻原生质体培养，日本晴、楚粳8号、云粳9号、大白谷和元江普通野生稻先后获得了原生质体培养的成功，除日本晴外，其余品种均为首次报道，品种间绿苗再生率有显著差异。日本晴最高，大白谷最低，同一品种不同的俞伤组织间，分化率有很大差异。有的愈伤组织极易分化出绿苗，有的不能再生出绿苗，说明分化前的预培养非常重要。N6分化培养基的再生绿苗率极显著地高于MS。配方为N6 葡萄糖20g/L 蔗糖30g/L IAA0.2mg/L 6-BA0.5mg/L Agarose-110g?L的培养基。分化率最高，按初次接种的愈伤组织块计，每块平均再生绿苗1.4苗,酶解原生质体量的多少,与愈伤组织在N6 1mg/L2,4-D的液体培养基中悬浮培养时间的长短有关,悬浮时间较长则易于获得较多的原生质体.日本晴的再生植株中,有两株多移栽到抽穗仅45d,有可能是极早良的变异株,有些植株的粒型和稃毛也有明显变异对鉴定和利用变异的植株具有现实意义. |
| 关 键 词： | 原生质体培养 绿苗再生率 水稻 普通野生稻 植株再生 |
| 文章编号： | 1001－4829(2001)01－0016－05 |
Plant regeneration from protoplasts of rice varieties in Yunnan |
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| Abstract: | Green plantlets were successfully gotten from protopl asts ofYunnan rice varieties,Chujing 8,Yunjing 9,Dabaigu and Yuanjiang common w ild rice and a Japanese variety,Nipponbare.Regeneration frequencies(RF) of green plantlets among varieties showed significant difference.Within one variety,some calli seemed to be very easily regenerated while other calli were difficult to g et green plantlets in spite of using many kinds of media.RF in medium N6 was muc h higher than that in medium MS.The yields of protoplasts were related to the ag es of suspension culture.Some plants derived from protoplast of Nipponbare showe d different seed shape,glume hairiness and mature time from the original plants. In these plants,genetic variation might have been induced during the period of in vitro culture. |
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