| 巨桉EgrWOX5基因的克隆、表达分析与超表达载体构建 |
| |
| 引用本文： | 徐双,曾炳山,范春节.巨桉EgrWOX5基因的克隆、表达分析与超表达载体构建[J].热带作物学报,2014,35(10):1957-1962. |
| |
| 作者姓名： | 徐双 曾炳山 范春节 |
| |
| 作者单位： | 中国林业科学研究院热带林业研究所,广东广州,510520 |
| |
| 基金项目： | 国家“863”项目“桉树与鹅掌楸转基因育种技术研究”（No. 2013AA100705）；中央级公益性科研院所基本科研业务费专项资金“桉树木材形成相关基因筛选与初步功能鉴定”（No. RITFYWZX201304）。 |
| |
| 摘 要： | 以巨桉(Eucalyptus grandis)EG5无性系为试验材料,通过RT-PCR技术从中克隆得到了1个巨桉WOX家族基因成员的CDS序列,命名为Egr WOX5(Gen Bank登录号为KF964019)。该基因全长为543 bp,编码181个氨基酸,具有WOX基因家族最保守的特征结构域-Homeobox domain(HD)结构域;其氨基酸序列与草本植物的拟南芥At WOX5基因和木本植物杨树Ptr WOX5基因的氨基酸序列有较高的相似性。组织表达分析结果显示,Egr WOX5基因特异性地在巨桉EG5的根组织中表达。这些结果间接或直接地说明Egr WOX5在巨桉根组织的发育过程中发挥重要作用。本研究还利用Gateway技术成功构建了Egr WOX5基因的超表达载体,为后续基因功能的进一步鉴定奠定基础。
|
| 关 键 词： | 巨桉 WOX5 基因克隆 表达分析 超表达载体构建 |
Expression Analysis and Molecular Cloning of WOX5 in Eucalyptus grandis and Over-expression Vector Constructed |
| |
| XU Shuang,ZENG Bingshan and FAN Chunjie.Expression Analysis and Molecular Cloning of WOX5 in Eucalyptus grandis and Over-expression Vector Constructed[J].Chinese Journal of Tropical Crops,2014,35(10):1957-1962. |
| |
| Authors: | XU Shuang ZENG Bingshan and FAN Chunjie |
| |
| Institution: | Research Institute of Tropical Forestry, Chinese Academy of Forestry;Research Institute of Tropical Forestry, Chinese Academy of Forestry;Research Institute of Tropical Forestry, Chinese Academy of Forestry |
| |
| Abstract: | A member of WOX gene family, EgrWOX5(Genbank Accession Number is KF964019)was cloned from Eucalyptus grandis using RT-PCR, which had the most conserved domain-Homeobox domain. The gene is 543 bp and encodes 181 amino acids. The deduced amino acid sequence of EgrWOX5 shares high similarity with AtWOX5 from Arabidopsis thaliana and PtrWOX5 from Populus trichocarpa. Expression analysis revealed its tissue-specific pattern in root. In conclusion, all the results could directly or indirectly prove EgrWOX5 play important roles in the development of root in E. grandis. The study also took advantage of Gateway technology and successfully constructed the over-expression vector of EgrWOX5, which would lay a foundation for further functional study of the gene. |
| |
| Keywords: | Eucalyptus grandis WOX5 Molecular cloning Expression analyses Over-expression vector construct |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
