| 大白菜核基因雄性不育复等位基因Ms的SSR标记 |
| |
| 引用本文： | 冯辉,魏鹏,李承彧,崔水莲,林容杓,朴钟云.大白菜核基因雄性不育复等位基因Ms的SSR标记[J].园艺学报,2009,36(1):103-108. |
| |
| 作者姓名： | 冯辉 魏鹏 李承彧 崔水莲 林容杓 朴钟云 |
| |
| 作者单位： | ( 沈阳农业大学园艺学院,沈阳 110161; 忠南大学基因组研究中心,韩国大田 305-764) |
| |
| 基金项目： | 国家自然科学基金,国家高技术研究发展计划(863计划),韩国农林水产食品部基金 |
| |
| 摘 要： | 利用大白菜细胞核复等位基因型雄性不育两用系‘AB01’的不育株,与多国芸薹属植物基因组计划MBGP的基础材料'Chiifu'杂交及回交,构建BC1群体。根据SSR检测体系中各主要成份浓度对扩增结果的影响,优化反应体系。选用250对SSR引物,对大白菜核基因雄性不育复等位基因Ms进行SSR+BSA分析,获得了7对多态性引物。在101株BC1个体间对这些引物进一步检测,得到2个与Ms基因连锁的SSR标记cnu_m273和cnu_m295。经连锁分析,二者位于Ms的同一侧,遗传距离分别为4.95 cM和7.92 cM。
|
| 关 键 词： | 大白菜 核基因雄性不育 复等位基因 SSR |
| 收稿时间： | 2008-10-15 |
Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene in Chinese Cabbage |
| |
| Feng Hui,Wei Peng,Li Cheng-yu,Choi Sh Ryun,Lim Yong Pyo,Piao Zhong-yun.Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene in Chinese Cabbage[J].Acta Horticulturae Sinica,2009,36(1):103-108. |
| |
| Authors: | Feng Hui Wei Peng Li Cheng-yu Choi Sh Ryun Lim Yong Pyo Piao Zhong-yun |
| |
| Institution: | (Department of Horticulture, Shenyang Agricultural University, Shenyang 110161; Plant Genome Research Center, Chungnam National University, Daejeon, 305-764, South Korea) |
| |
| Abstract: | A BC1 mapping population was constructed with Chinese cabbage inbred line "Chiifu" which is the model material of Multinational Brassica Genome Project as the male parent and genic multiple allele male sterile material 'AB01' as the female parent. Major effects of components in SSR-PCR system on amplification result were probed. 250 SSR primer pairs were employed to identify SSR markers linked to the genic multiple allele male sterile gene "Ms". Within the eight SSR primer pairs showing polymorphism between two gene bulks, two SSR markers named cnu_m273 and cnu_m295 were found linked to the Ms gene, at the genetic distances of 4.95 cM and 7.92 cM respectively. |
| |
| Keywords: | SSR |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
