| 广西家蚕核型多角体病毒株gp64基因克隆与序列分析 |
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| 引用本文： | 陈朝蓉,屈达才,朱方容,李俊,闭立辉,梁湘.广西家蚕核型多角体病毒株gp64基因克隆与序列分析[J].南方农业学报,2017,48(2). |
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| 作者姓名： | 陈朝蓉 屈达才 朱方容 李俊 闭立辉 梁湘 |
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| 作者单位： | 1. 广西大学 农学院,南宁,530004;2. 广西蚕业科学研究院,南宁,530007;3. 广西大学 农学院, 南宁 530004;亚热带农业生物资源保护与利用国家重点实验室, 南宁 530004 |
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| 基金项目： | 广西科学研究与技术开发计划项目 |
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| 摘 要： | 【目的】了解广西家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus,Bm NPV)毒株gp64基因的保守性,并掌握其具体的突变位点,为研究核型多角体病毒(NPV)种内的微进化模式提供参考依据。【方法】对广西不同桑蚕产区的19株Bm NPV毒株进行gp64基因克隆与测序,分析完整开放阅读框(ORF)序列的同源性和单核苷酸多态性,并构建基于gp64基因的遗传进化树。【结果】广西Bm NPV毒株的gp64基因ORF序列不一致,存在1590、1593和1599 nt三种类型,是由于第94~96位缺失GCG或第97~102位插入GCGCCG所致,且插入核苷酸突变仅在广西Bm NPV毒株中出现。仅GXSL毒株与T3毒株的核苷酸及其推导氨基酸序列同源性均达100.0%,其余18株毒株与T3毒株的核苷酸序列同源性在98.5%~99.2%,其推导氨基酸序列同源性在98.7%~99.6%。19株广西Bm NPV毒株被分为两个亚群(cladeⅠ和cladeⅡ),其中11株独立形成cladeⅡ,7株独立形成cladeⅠ中的一个亚群;广西毒株在多个位点出现同义核苷酸突变,呈一定的规律性。【结论】广西Bm NPV毒株的gp64基因具有遗传多样性,在遗传进化上较独立,插入突变显示出地域特点。
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| 关 键 词： | 家蚕核型多角体病毒(BmNPV) gp64基因 克隆 序列分析 微进化 广西 |
Cloning and sequence analysis of gp64 gene in Bombyx mori nucleopolyhedrovirus Guangxi isolates |
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| CHEN Chao-rong,QU Da-cai,ZHU Fang-rong,LI Jun,BI Li-hui,LIANG Xiang.Cloning and sequence analysis of gp64 gene in Bombyx mori nucleopolyhedrovirus Guangxi isolates[J].Journal of Southern Agriculture,2017,48(2). |
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| Authors: | CHEN Chao-rong QU Da-cai ZHU Fang-rong LI Jun BI Li-hui LIANG Xiang |
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| Abstract: | Objective]This research aimed to understand gp64 gene conservation of Bombyx mori nucleopolyhedrovirus (BmNPV) Guangxi isolates and master the specific site of mutation, which could provide reference for study of intraspecific microevolution pattern of nucleopolyhedrovirus(NPV). Method]The gp64 genes of 19 BmNPV strains collected from dif-ferent sericultural areas in Guangxi were cloned and sequenced. Homology and the mononucleotide polymorphisms of gp64 complete open reading frame(ORF) were analyzed, and phylogenetic tree of gp64 gene was established. Result]The gp64 gene ORF sequences of Guangxi BmNPV strains were not consistent, showing three types as 1590 nt, 1593 nt and 1599 nt. The diversities were caused by GCG deletion in position 94-96, and GCGCCG insertion in position 97-102. inser-tion mutations of nucleotide only happened in Guangxi BmNPV strains. Only GXSL strain shared 100.0%homologies in nu-cleotide and deduced amino acid sequence with T3 strain. The other 18 strains shared 98.5%-99.2% homology in nu-cleotide sequence and 98.7%-99.6% homology in deduced amino acid sequence with T3 strain. All 19 Guangxi BmNPV strains were separated into two clades(clade Ⅰand clade Ⅱ). Among them, 11 strains were included in clade Ⅱ sep-arately and 7 strains were included in a sub-clade of cladeⅠ. Synonymous substitutions occurred in several positions of gp64 genes from Guangxi BmNPV strains, showing certain regularity.Conclusion]The gp64 genes of Guangxi BmNPV strains em-bodies genetic diversity and independent genetic evolution. The insertion mutation indicates territorial characteristic. |
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| Keywords: | Bombyx mori nucleopolyhedrovirus ( BmNPV ) gp64 gene clone sequence analysis microevolution Guangxi |
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