| 吉林省肉牛冠状病毒感染状况调查及分析 |
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| 引用本文： | 孙飞雁,叶京飞,魏宇,王子贤,张金玉,邴丽媛,孟婷婷,王帅,赵立峰,孙亮,郭利.吉林省肉牛冠状病毒感染状况调查及分析[J].畜牧兽医学报,2023,54(2):673-682. |
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| 作者姓名： | 孙飞雁 叶京飞 魏宇 王子贤 张金玉 邴丽媛 孟婷婷 王帅 赵立峰 孙亮 郭利 |
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| 作者单位： | 1. 吉林农业科技学院, 吉林 132101;2. 中国农业科学院特产研究所, 长春 130122;3. 吉林农业大学动物科学技术学院, 长春 130118;4. 吉林大学动物科学学院, 长春 130012;5. 吉林省动物疫病预防控制中心, 长春 133000 |
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| 基金项目： | 吉林省科技厅吉林省科技发展计划项目（20210202101NC）；吉林农业科技学院博士启动基金项目（吉农院合字第[2021]7008号） |
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| 摘 要： | 旨在全面了解牛冠状病毒(BCoV)在吉林省肉牛群的流行情况，选择吉林省东中西部地区的12个县市的规模化、养殖合作社、家庭型养殖户等牛场在不同季节采集血液、鼻拭子、粪拭子及临床病死牛组织脏器，采用血清学及分子生物学诊断检测技术，对BCoV进行流行病学调查，了解BCoV在该吉林省部分地区的流行情况。共采集临床血清样品1 298份，粪便样品、肝、肺、脾、气管等组织样品462份，应用商品化BCoV抗体检测试剂盒检测血清抗体和纳米PCR新型检测技术对临床样品进行PCR检测，并对核酸检测出的阳性结果测序分析。结果显示BCoV抗体血清阳性率为1.08%,粪便、肝等临床样品阳性率21.10%。经测序分析调查地区BCoV流行株与我国四川流行株相似性达99%以上。本研究对吉林省中部地区BCoV流行情况进行了全面调查，丰富了牛冠状病毒的流行病学调查数据，为指导牛冠状病毒的防控工作奠定了基础。
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| 关 键 词： | 牛冠状病毒 流行病学调查 |
| 收稿时间： | 2022-05-12 |
Epidemiological Investigation and Analysis of Bovine Coronavirus in Beef Cattle in Jilin Province |
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| SUN Feiyan,YE Jingfei,WEI Yu,WANG Zixian,ZHANG Jinyu,BING Liyuan,MENG Tingting,WANG Shuai,ZHAO Lifeng,SUN Liang,GUO Li.Epidemiological Investigation and Analysis of Bovine Coronavirus in Beef Cattle in Jilin Province[J].Acta Veterinaria et Zootechnica Sinica,2023,54(2):673-682. |
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| Authors: | SUN Feiyan YE Jingfei WEI Yu WANG Zixian ZHANG Jinyu BING Liyuan MENG Tingting WANG Shuai ZHAO Lifeng SUN Liang GUO Li |
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| Institution: | 1. Jilin Agricultural Science and Technology University, Jilin 132101, China;2. Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130122, China;3. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;4. Animal Sciences College, Jilin University, Changchun 130012, China;5. Jilin Animal Epidemic Disease Prevention and Control Center, Changchun 133000, China |
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| Abstract: | In order to comprehensively understand the epidemiological situation of bovine coronavirus (BCoV) in beef cattle herds in Jilin Province, blood, nasal swabs, fecal swabs and tissue organs of clinically diseased and dead cattle were collected in different seasons from 12 counties and cities in the east, central and western regions of Jilin Province, using serological and molecular diagnostic testing techniques to conduct an epidemiological investigation of BCoV in the The epidemiological situation of BCoV in some areas of Jilin Province. A total of 1 298 clinical serum samples, 462 clinical samples (including fecal samples, liver, lung, spleen, trachea and other tissue samples) were collected, and PCR detection of clinical samples was performed by applying commercial BCoV antibody detection kits to detect serum antibodies and a novel detection technique of nano-PCR, and sequencing and analysis of positive results detected by nucleic acid. The results showed that the serum positive rate of BCoV antibodies was 1.08%, and the positive rate of clinical samples such as feces and liver was 21.10%. The BCoV prevalent strain in the investigated area was more than 99% homologous to the prevalent strain in Sichuan, China, after sequencing analysis. This study provides a comprehensive survey of BCoV prevalence in central Jilin Province, which enriches the epidemiological survey data of bovine coronavirus and lays the foundation for guiding the prevention and control of bovine coronavirus. |
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| Keywords: | bovine coronavirus epidemiological investigation |
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