| 6-戊基-2H-吡喃-2-酮对草坪币斑病菌的抑菌活性及其对病害的防治效果 |
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| 引用本文： | 刘曼,牛启尘,尹淑霞,王子玥.6-戊基-2H-吡喃-2-酮对草坪币斑病菌的抑菌活性及其对病害的防治效果[J].农药学学报,2023,25(1):104-116. |
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| 作者姓名： | 刘曼 牛启尘 尹淑霞 王子玥 |
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| 作者单位： | 1.北京林业大学 草业与草原学院，北京 100083 |
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| 基金项目： | 国家林业和草原局委托项目 (2021045001)；国家自然科学基金 (U20A2005). |
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| 摘 要： | 由Clarireedia spp.引起的草坪币斑病是对草坪最具有破坏性的病害之一，6-戊基-2H-吡喃(6-pentyl-2H-pyran-2-one, 6PP)是木霉菌属(Trichoderma spp.)重要的抗菌次生代谢产物。为探究6PP对草坪币斑病菌的抑菌活性、防治效果以及草坪币斑病菌对6PP的生理响应，本研究采用菌丝生长速率法测定了6PP对草坪币斑病菌的抑制活性，分别采用室内离体叶片和盆栽试验测定了6PP对草坪币斑病的预防和治疗作用，并分析了在6PP影响下草坪币斑病菌菌丝结构及抗逆相关酶活性的变化。结果显示，6PP对两种草坪币斑病菌C. jacksonii和C. monteithiana均具有高抑制活性，其中对C. monteithiana的平均有效抑制中浓度(EC50)为0.37 μg/mL，对C. jacksonii的EC50值为0.04 μg/mL；经6PP处理后，C. jacksonii和C. monteithiana菌丝生长异常，细胞膜通透性增加，相对电导率上升，细胞膜脂过氧化，细胞膜受损；C. jacksonii过氧化物酶(POD)的活性显著上升，但pH值不变，而C. monteithiana超氧化物歧化酶(SOD)活性、pH值和胞外多糖(EPS)显著上升。1 μg/mL的6PP在离体条件下对币斑病的预防效果为91.14%；盆栽条件下，防治效果为72.21%。6PP对草坪币斑病菌抑制作用显著，且在离体和盆栽条件下对病害均有良好的防治效果，具备开发成生态友好型杀菌剂的潜力。
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| 关 键 词： | 草坪币斑病菌 6-戊基-2H-吡喃 抑菌活性 草坪币斑病 防治效果 病原生理响应 |
| 收稿时间： | 2022-03-18 |
Inhibitory activity of 6-pentyl-2H-pyran-2-one against the pathogenesis fungi of dollar spot and its control efficacy |
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| Institution: | 1.School of Grassland Science, Beijing Forestry University, Beijing 100083, China |
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| Abstract: | Turf dollar spot, caused by Clarireedia spp., is one of the most destructive fungal diseases on turf. 6-Pentyl-2H-pyran-2-one (6PP) is a critical antifungal secondary metabolite produced by Trichoderma spp. In order to explore the antifungal activity of 6PP against Clarireedia spp. and its control efficacy to Turf dollar spot, the inhibition activity of 6PP on Clarireedia spp. was measured by mycelial growth rate method, and the protective and curative activities of 6PP against the disease was measured on detached leaves and potted plants. The changes in mycelial structure and activities of stress resistance related enzyme of the pathogens under 6PP treatment were analyzed. The results showed that 6PP had efficient inhibition activity on C. jacksonii and C. monteithiana. The average effective inhibitory medium concentration (EC50) of 6PP to C. monteithiana was 0.37 μg/mL, and the average EC50 value to C. jacksonii was 0.04 μg/mL. After being treated with 6PP, mycelial of both C. jacksonii and C. monteithiana grew abnormally, cell membrane permeability and relative conductivity as well as peroxidated cell membrane increased. The peroxidase (POD) activity of C. jacksonii increased significantly although its pH remained unchanged, while the superoxide dismutase (SOD) activity, pH and exopolysaccharides (EPS) of C. monteithiana increased significantly. The preventive effects of 1 μg/mL 6PP on dollar spot were 91.14% in vitro and 72.21% in pots. 6PP has a significant inhibitory effect on Turf dollar spot and has a good control efficacy on the disease in vitro and in pots. The study revealed the potential of 6PP to be an eco-friendly fungicide preventing Turf dollar spot. |
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