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| 华南三省(自治区)部分西番莲种植区花叶病病原检测及EAPV多聚蛋白基因序列分析 | |
| 引用本文： | 叶婷,郑国华,孟红岩,张越华,赖瑞云,郭莺.华南三省(自治区)部分西番莲种植区花叶病病原检测及EAPV多聚蛋白基因序列分析[J].植物病理学报,2023,53(1):151-154. |
| 作者姓名： | 叶婷 郑国华 孟红岩 张越华 赖瑞云 郭莺 |
| 作者单位： | 福建省亚热带植物研究所/福建省亚热带植物生理生化重点实验室, 厦门 361006; 厦门华侨亚热带植物引种园/厦门市植物引种检疫与植物源产物重点实验室,厦门 361002; 厦门大学实验动物中心,厦门 361004 |
| 基金项目： | 厦门市东西协作科技扶贫项目(3502Z20204503-2);厦门市科技计划项目(3502Z20162013) |
| 摘 要： | <正>西番莲(Passiflora edulis Sims.)是热带亚热带地区重要经济作物，我国福建、广东、广西、海南等地广泛种植1]。调查发现，我国南方多地西番莲花叶病为害严重，症状表现为叶片花叶、黄化、斑驳、畸形，甚至整株褪绿，严重影响西番莲的产量和品质。已报道能引起西番莲花叶症状的病毒有：黄瓜花叶病毒属(Cucumovirus)的黄瓜花叶病毒(cucumber mosaic virus, CMV), |
| 关 键 词： | Passiflora edulis mosaic disease cucumber mosaic virus Potyvirus East Asian passiflora virus molecular detection |
| 收稿时间： | 2021-10-24 |
Viral pathogens detection of Passiflora edulis mosaic disease and sequence analysis of polyprotein gene of East Asian passiflora virus in some areas of South China |
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| YE Ting,ZHENG Guohua,MENG Hongyan,ZHANG Yuehua,LAI Ruiyun,GUO Ying.Viral pathogens detection of Passiflora edulis mosaic disease and sequence analysis of polyprotein gene of East Asian passiflora virus in some areas of South China[J].Acta Phytopathologica Sinica,2023,53(1):151-154. | |
| Authors: | YE Ting ZHENG Guohua MENG Hongyan ZHANG Yuehua LAI Ruiyun GUO Ying |
| Institution: | Fujian Institute of Subtropical Botany /Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Xiamen 361006, China; Xiamen Overseas Chinese Subtropical Plant Introduction Garden / Plant Introduction & Quarantine Base and Plant Pro-duct Key Laboratory of Xiamen City, Xiamen 361002, China; Xiamen University Laboratory Animal Center, Xiamen 361004, China |
| Abstract: | Passiflora edulis mosaic disease is a serious constraint to the production of Passiflora edulis. in China. During 2016-2019, 170 samples of P. edulis with typical mosaic symptoms were collected from 7 planting areas in Fujian, Guangxi and Guangdong. Cucumber mosaic virus (CMV), Potyvirus and Tobamovirus were detected using RT-PCR with degenerate primers and/or specific primers. The results showed that 90 out of 170 samples were detected positive. The detection rate of CMV was 51.18% while the detection rate of Potyvirus was 10.00 %, however, Tobamovirus was not found in this research. CMV and Potyvirus showed severe mixed infection which account for 15.56% of total infection rate. Further identification of viruses in the genus Potyvirus was performed and the amplified fragment shared 98.00%-100.00% identity with East Asian passiflora virus aviliable in the GenBank. Additionally, 5 isolates with polyprotein gene sequences were obtained and phylogenetic analysis of these 5 isolates indicated a high genetic relationship to the AO stain reported from Japan and China (Taiwan). These results will be helpful for the detection and effective control of Passiflora edulis mosaic disease. |
| Keywords: | Passiflora edulis mosaic disease cucumber mosaic virus Potyvirus East Asian passiflora virus molecular detection |
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