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脱支和反复湿热处理对苦荞抗性淀粉含量和理化特性的影响
引用本文:付梓平,范昱,赖弟利,张凯旋,朱剑锋,李基光,周美亮,王俊珍.脱支和反复湿热处理对苦荞抗性淀粉含量和理化特性的影响[J].作物杂志,2023,39(1):52-450.
作者姓名:付梓平  范昱  赖弟利  张凯旋  朱剑锋  李基光  周美亮  王俊珍
作者单位:1成都大学食品与生物工程学院,610000,四川成都2中国农业科学院作物科学研究所,100081,北京3贵州大学农学院,550000,贵州贵阳4凉山彝族自治州农业科学研究院,615000,四川凉山5湖南省农业科学院作物研究所,410000,湖南长沙
基金项目:国家重点研发计划(2020YFD1000800);国家重点研发计划(2020YFD1000802-01);河北省教育厅青年拔尖人才项目(BJ2019022)
摘    要:采用普鲁兰酶(PUL)去除苦荞淀粉支链,进行多次湿热处理(HMT),探究PUL用量和HMT次数对苦荞全粉中抗性淀粉含量以及理化特性的影响。结果表明,PUL、HMT和PUL-HMT均能增加苦荞抗性淀粉含量,其中PUL用量10U/g、HMT 2次效果最佳,抗性淀粉含量从4.74%增加到13.38%。经过PUL-HMT,苦荞粉表面由光滑变凹凸状,晶型结构发生变化,从A型变为A+V型,相对结晶度和热焓值下降,红外光谱表明,淀粉内部短程有序结构轻微改变。去支化和HMT能有效改变苦荞粉理化性质,增加抗性淀粉含量,其中PUL10-HMT2的处理方法能得到更加理想的高抗性淀粉苦荞材料。

关 键 词:苦荞  普鲁兰酶  湿热处理  抗性淀粉  理化性质
收稿时间:2022-06-21

Effects of Debranched and Repeated Wet Heat Treatment on Content and Physicochemical Properties of Resistant Starch of Tartary Buckwheat
Fu Ziping,Fan Yu,Lai Dili,Zhang Kaixuan,Zhu Jianfeng,Li Jiguang,Zhou Meiliang,Wang Junzhen.Effects of Debranched and Repeated Wet Heat Treatment on Content and Physicochemical Properties of Resistant Starch of Tartary Buckwheat[J].Crops,2023,39(1):52-450.
Authors:Fu Ziping  Fan Yu  Lai Dili  Zhang Kaixuan  Zhu Jianfeng  Li Jiguang  Zhou Meiliang  Wang Junzhen
Abstract:Starch branch chains of Fagopyrum tataricum were removed by pullulanase (PUL), and the effects of PUL dosage and times of wet and heat treatment (HMT) on resistant starch content and physicochemical properties of F.tataricum whole powder were investigated. The results showed that the content of resistant starch of F.tataricum could be increased by PUL, HMT and PUL-HMT, in which the dosage of PUL was 10U/g (PUL10), and the two times treatment (HMT2) had the best effects, the resistant starch content increased from 4.74% to 13.38%. After PUL-HMT, the surface of F.tataricum powder changed from smooth to concave and convex, the crystal structure changed from A type to A+V type, and the relative crystallinity and enthalpy value decreased. Infrared spectroscopy showed that the short-range ordered structure in starch changed slightly. The chemical properties and resistant starch content of F.tataricum powder could be effectively changed by debranching and HMT, and PUL10-HMT2 treatment could get more ideal F.tataricum material with high resistant starch.
Keywords:Fagopyrum tataricum  Pullulanase  Wet heat treatment  Resistant starch  Physicochemical properties  
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