| 乳制品中A1 β-酪蛋白、A2 β-酪蛋白检测方法研究进展 |
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| 引用本文： | 袁兆丰,杨秀梅,王丹,王青云,车佳玲.乳制品中A1 β-酪蛋白、A2 β-酪蛋白检测方法研究进展[J].中国乳业,2023,0(3):80-84. |
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| 作者姓名： | 袁兆丰 杨秀梅 王丹 王青云 车佳玲 |
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| 作者单位： | 北大荒完达山乳业股份有限公司,黑龙江哈尔滨 150000 |
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| 基金项目： | 黑龙江省“百千万”工程科技重大专项-超高端有机A2系列乳制品研制开发及产业化示范（2021ZX12B01） |
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| 摘 要： | 随着消费者对A2 β-酪蛋白的关注度提升,A2 β-酪蛋白相关产品不断更新换代。A2 β-酪蛋白常用检测方法有反相高效液相色谱法（RP-HPLC）、液相色谱–高分辨串联质谱法、毛细管区带电泳法、试剂盒检测法等。本文对β-酪蛋白遗传变体A1 β-酪蛋白和原始形态A2 β-酪蛋白的功能特点、检测方法、定量分析进行综述,说明检测方法的原理和试剂药品的作用机理,加强对A1 β-酪蛋白、A2 β-酪蛋白检测方法的改进与优化,为A2 β-酪蛋白应用于功能性食品、婴幼儿配方食品提供理论依据。
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| 关 键 词： | A1β-酪蛋白 A2β-酪蛋白 反相高效液相色谱法 液相色谱–高分辨串联质谱法 毛细管区带电泳法 快速试剂盒检测法 |
Research Progress in Detection Methods of A1 β-Casein and A2 β-Casein in Dairy Products |
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| YUAN Zhaofeng,YANG Xiumei,WANG Dan,WANG Qingyun,CHE Jialing.Research Progress in Detection Methods of A1 β-Casein and A2 β-Casein in Dairy Products[J].China Dairy,2023,0(3):80-84. |
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| Authors: | YUAN Zhaofeng YANG Xiumei WANG Dan WANG Qingyun CHE Jialing |
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| Institution: | Bei Da Huang Wandersun Dairy Holdings Co.,Ltd.,Harbin Heilongjiang 150000 |
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| Abstract: | With consumers' concern for increased attention to A2 β-casein,A2 β-Casein related products are also constantly updated, Common detection methods for A2 β- casein include,reversed phase high performance liquid chromatography （RP-HPLC）, liquid chromatography high resolution tandem mass spectrometry,capillary zone electrophoresisrapid kit detection,etc.. This paper reviewed the functional characteristics,detection methods and quantitative analysis of β-Casein genetic variant A1 β-Casein and A2 β-casein in original form. The purpose is to explain the principle of detection method and the action mechanism of reagents and drugs,strengthen the improvement and optimization of A1 β-Casein and A2 β-casein detection method. It provided theoretical basis for the application of A2 β-casein in functional food,including milk base material and infant formula food. |
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| Keywords: | A1 β-casein A2 β-casein reversed phase high performance liquid chromatography liquid chromatography high resolution tandem mass spectrometry capillary zone electrophoresis rapid kit detection |
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