Interleukin-2 and interleukin-10 gene expression in calves experimentally infected with Fasciola gigantica |
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| Authors: | SL Ingale P Singh OK Raina AK Verma R Channappanavar UR Mehra |
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| Institution: | 1. Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar, 243 122, India;2. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122, India;3. Department of Biological Sciences, Oakland University, Rochester Hills, MI- 48309, USA |
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| Abstract: | The gene expression of interleukin-2 and interleukin-10 in calves with a primary infection of Fasciola gigantica was studied. Five calves were infected orally with a dose of 1000 viable metacercariae of F. gigantica and five calves served as uninfected control animals. Expression of two cytokine genes i.e. IL-2 and IL-10 (Th1 and Th2) was measured at 10, 30 and 75 days post-infection (PI) by real-time polymerase chain reaction with the double stranded DNA-binding dye SYBR Green. Interleukin-2 was not detected in the peripheral blood mononuclear cells (PBMCs) of infected or control animals at 10, 30 and 75 days PI, however, IL-10 was present in detectable levels in PBMCs of infected animals at 10, 30 and 75 days PI, with no expression of this cytokine in the control animals. With an increased expression of IL-10 and no expression of IL-2 cytokine gene, the present study suggests that F. gigantica infection in calves evoked Th2 immune response. |
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| Keywords: | Cattle Fasciola gigantica IL-2 IL-10 Real-time PCR |
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