| 猪传染性胃肠炎病毒的分离鉴定 |
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| 引用本文： | 陈焕春,刘超.猪传染性胃肠炎病毒的分离鉴定[J].华中农业大学学报,1992,11(1):82-86. |
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| 作者姓名： | 陈焕春 刘超 |
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| 作者单位： | 华中农业大学畜牧兽医系，华中农业大学畜牧兽医系，华中农业大学畜牧兽医系，华中农业大学畜牧兽医系，华中农业大学畜牧兽医系 武汉 430070，武汉 430070，武汉 430070，武汉 430070，武汉 430070 |
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| 摘 要： |
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| 关 键 词： | 病毒分离 猪 传染性 胃肠炎 |
ISOLATION AND IDENTIFICATION OF THE TRANSMISSIBLE GASTROENTERITIS VIRUS FROM PIGS |
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| CHEN Huan-chun LIU Chao JIN Mei-lin LUO Man-lin WU Mei-zhou.ISOLATION AND IDENTIFICATION OF THE TRANSMISSIBLE GASTROENTERITIS VIRUS FROM PIGS[J].Journal of Huazhong Agricultural University,1992,11(1):82-86. |
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| Authors: | CHEN Huan-chun LIU Chao JIN Mei-lin LUO Man-lin WU Mei-zhou |
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| Abstract: | Among six pig farms had an outbreak of severe diarrhea. Samples of mesenteric lymph gland and tonsil from those moribund and died piglet were examined by immuno-fluo-rescent technique (IFAT) for detection of transmissible gastro- enteritic (TGE) viral antigen. The IFAT strong positive mesenteric lymph gland were used as inoculum. It were inoculated onto the primary pig thyroid cell monolayer and found with marked cell pathological effect (CPE) affter three successive passages. Until gth passages, the virus adapted successfully to a continuous pig kidney cell line IBRS-2. A strain of those isolates which had typical CPE was identified as TGE virus by morphological observation with electro-microscope, by physiochemical assaies and by serological tests. |
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| Keywords: | transmissible gastro-enteritios virus isolation neutralization test pig |
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