| 铁皮石斛phytocyanin基因家族全基因组分析 |
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| 引用本文： | 宋争,李潞滨,梁立雄,王涛.铁皮石斛phytocyanin基因家族全基因组分析[J].林业科学研究,2018,31(2):98-106. |
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| 作者姓名： | 宋争 李潞滨 梁立雄 王涛 |
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| 作者单位： | 林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所 |
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| 基金项目： | 国家高技术研究发展计划（863计划）子课题（2013AA102607-3）；‘十二五’农村领域国家科技支撑计划子课题（2012BAD01B0702） |
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| 摘 要： | 目的]探讨Phytocyanins(PCs)在铁皮石斛发育过程和逆境胁迫环境下的潜在功能。方法]利用拟南芥和水稻phytocyanin基因家族蛋白序列(At PCs和Os PCs),采用本地化软件BLASTP对铁皮石斛全基因组数据库进行搜索,并采用SMART和pfam数据库验证质体蓝素样结构域,获得铁皮石斛phytocyanin基因家族编码序列(Do PCs);利用Signal P4.1、Big-PI Plant Predictor、Net NGlyc1.0 Server等在线软件对Do PC家族序列的结构特点进行分析;利用软件Clustal W和MEGA对Do PC基因进行氨基酸序列的比对和系统进化分析;利用转录组数据绘制heatmap图对Do PCs基因在与美孢胶膜菌共生萌发的种子中的表达情况进行分析。结果]显示:铁皮石斛全基因组中共预测到包含PCLD结构域的38个Do PCs,可分属4个亚家族,2个Cys残基在Do PCs家族中高度保守;19条Do PCs蛋白序列含有4个完整的保守铜结合配体(His、Cys、His、Gln/Met),有32条Do PCs骨架中含有N-端信号肽,22条Do PCs含有糖基磷脂酰肌醇锚定位点,有27条Do PCs含N-糖基化位点;16个Do PCs基因在与美孢胶膜菌共生萌发的种子中检测到表达,其中,Do UCL2,4和Do ENODL14基因表达量最高,7个基因在共生萌发的种子中明显上调表达,只有Do ENODL6基因明显下调表达。结论]在全基因组范围内分析铁皮石斛Do PC家族蛋白结构特点,可以为探究兰科植物与微生物共生的相互作用及进一步研究兰科菌根形成的分子机制提供数据基础。
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| 关 键 词： | 铁皮石斛 phytocyanin基因家族 全基因组分析 共生萌发种子 |
| 收稿时间： | 2016/11/4 0:00:00 |
Genome-wide Analysis of Phytocyanin Gene Family in Dendrobium officinale |
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| SONG Zheng,LI Lu-bin,LIANG Li-xiong and WANG Tao.Genome-wide Analysis of Phytocyanin Gene Family in Dendrobium officinale[J].Forest Research,2018,31(2):98-106. |
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| Authors: | SONG Zheng LI Lu-bin LIANG Li-xiong and WANG Tao |
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| Institution: | State Key Laboratory of Tree Genetic and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China,State Key Laboratory of Tree Genetic and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China,State Key Laboratory of Tree Genetic and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China and State Key Laboratory of Tree Genetic and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China |
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| Abstract: | Objective] To study the sequence characteristics of Dendrobium officinale Method] Local BLASTP was used to search the phytocyanin gene of D. officinale(DoPCs) in the database of Dendrobium whole genome, and then the conserved Plastocyanin-like domain and structural characteristics of DoPCs were predicted. The sequence alignment and phylogenetic analysis of DoPCs were conducted by using Clustal W and MEGA. Finally, 38 DoPCs were expected to have conserved PCLD domains and two Cys residues, belonging to four subfamilies. Result] Among these DoPC proteins, 19 DoPCs contained four copper ligands (His, Cys, His, and Gln/Met), 32 DoPCs were predicted having N-terminal signal peptides, 22 DoPCs had putative C-terminal glycosylphosphatidylinositol-anchor signals, and 27 DoPCs had putative arabinogalactan glycomodules. The results of DoPCs expression analysis in symbiotic with Tulasnella calospora and asymbiotic germinated seeds from D. officinale by using RNA-seq data showed that 16 DoPCs were expressed in symbiotic germinated seeds, and DoUCL2, 4 and DoENODL14 had the highest expression with different levels. Seven DoPC genes were obviously up-regulated and only one was down-regulated in symbiotic germinated seeds comparing with that of asymbiotic. Conclusion] The study of the protein structure characteristics of DoPCs family in Dendrobium officinale during the whole genome, would help in researching the interaction between Orchidaceae and microorganism, furthermore would serve as data foundation, in researching the molecular mechanism of Orchid mycorrhiza growth. |
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| Keywords: | Dendrobium officinale phytocyanin gene family genome-wide analysis symbiotic germinated seeds |
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