Effect of Protein Hydrolysates from Germinated Soybean on Cancerous Cells of the Human Cervix: An <Emphasis Type="Italic">In Vitro</Emphasis> Study |
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Authors: | R Mora-Escobedo Maria del Carmen Robles-Ramírez Eva Ramón-Gallegos Rafael Reza-Alemán |
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Institution: | 1.Escuela Nacional de Ciencias Biológicas,México,México;2.Campo Experimental Iguala,INIFAP,México |
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Abstract: | Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination
on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of
soybean protein through generation of amino acids or bioactive peptides. Soybean was germinated for 0, 2, 3, 4, 5, and 6 days
and proteins were isolated from the seeds. Isolates with and without ethanol-soluble phytochemicals were hydrolyzed with digestive
enzymes and their effect on growth in HeLa and C-33 (epidermoid cervical carcinoma) and HaCaT (non-cancerous human keratinocytes)
cells were evaluated with the Alamar Blue method. Germination induced degradation of the α and α’ fractions of β-conglycinin
and acid fraction of glycinin, generating low molecular weight peptides. Degrees of hydrolysis ranged from 73–77%. Hydrolysates
inhibited the growth of HeLa cells and C-33 at concentrations exceeding 1.25 mg/ml. Major inhibition was observed with the
hydrolysate germinated for 2 days and containing ethanolsoluble phytochemicals (IC50 2.15 and 2.27 mg/ml for HeLa and C-33, respectively). Interestingly, hydrolysate cytoxicity for normal cells was minimal
in comparison to cancer cells. |
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